Nerve Growth Factor And Collagen, The Lif-ce Analysis Method And Its Application

Objective: In order to study the mechanism of spinal cord injury and repair, and to explore the change of brackets and culture cells, the analytical methods for two kinds of protein, namely nerve growth factor (NGF) and collagen by capillary electrophoresis (CE) with laser induced fluorescence (LIF) were to develop. The new technique of sanitary technology combines biochemistry and clinic research, which can provide a fast, sensitive, effective and automated method and the evidence for nerve growth factor (NGF) of clinic use and the selection of the optimum conditions for organization engineering.Methods: he influence of parameters on the determination of the NGF and collagen was investigated and the procedure carried out as follows:1. Purification of NGF NGF in spinal cord of rat was purified by Sodium Dodecyl Sulfate-Polyacrylamide Gel electrophoresis (SDS-PAGE), and then its purity was determined by HPLC and immunocompetence was detected by Western blot, respectively. After these necessary processes, The product can be provided to the CE-immunoassay method (used as antigen).2. LIF-capillary electrophoresis-based immunoassay (CEIA) method of NGF After dissolved in phosphate buffer solution (PBS) and concentrated by vacuum freezing dryness, NGF in spinal cord can react with monoclonal antibody of NGF labeled by FITC. Then the antigen-antibody compound,...