The Fermentation Of Anti-tumor Antibiotics, S-632-a <sub> 2 </ Sub>, A <sub> 3 </ Sub>, Extraction, Structure Identification And Differentiation Inducing Role

Tumor is one of the most serious diseases that damaged to the human being, so the research of tumor's prevention and cure is always the focus of research by the scientists of biology, medicine and pharmacology in recent years. The chemotherapy of tumor is one the three general therapeutics in clinic therapy, now chemotherapy is developed into many systems which includes alkylating agent, antimetabolite, biologic response regulator. Differentiation inducer, especially the discovery of retinoic acid, has give the new clew to cure tumors, but taking retinoic acid long time may make the acute promyelocytic leukemia(APL) less in clinics, so one side induction differentiation mechanism need be researched, and the other side scientists look for new differentiation-inducer constantly.We screened the differentiation-inducer from the metabolite of microorganism and found the fermentation broth of Streptomyces hygroscopicus S-632 which was collected in ShanDong Province of China had the obvious active that induce acute promyelocytic leukemia HL-60 deoxidize NBT. And found this active is parallel to the anti-yeast active of the fermentation broth too. We also make some primary research about the fermentation conditions.During the process of puring the stain's fermentation liqor, using Saccharomyces cerevisiae No. 30 as verifiable microorganism , extracting by EA, absorbing by Diaion HP-20, chromatographing by basic silica gel column chromatography, TLC, medium pressure liquid chromatography, preparative HPLC etc.. and detecting by TLC; UV; HPLC and biology detection. We got the S-632-A₂and S-632-A₃ from the fermentation broth, using UV, IR, SIMS, 1H-NMR, 13C-NMR, 1H-1H COSY, NOE, confirm S-632-A₂ is 9-Methylstreptimidone, it's a known antibiotics, S-632-A., ( 3-[(2R, 5S, 6E, 8E)-5, 7-dimethyl-4-oxo-2-hydroxy-6,8-dicadienyl]- glutarimade) is the isomeric compound of S-632-A₂ the two compounds' structural formula is shown as follows:...