Bone Marrow Mesenchymal Stem Cells In Acute Myocardial Infarction And Cardiovascular Disease When The Biological Characteristics Of Changes In Research

PART ONE Objective To test the potential of human marrow mesenchymal stem cells (MSCs) differentiating into cardiovascular tissue. Methods 5-azacytidine (5-aza) was used to induce MSCs 's differentiating into myocardial cells. MSCs were induced to differentiate into endothelial cells by VEGF-B. Isoproterenol was used to produce myocardial injury model in NOD/SCID mice. Labeled MSCs were injected into tail vein. Immunofluorescence was performed to evaluate MSCs differentiation in vitro and vivo. Results MSCs treated by 5-aza expressed cardiac myosin heavy chain and cardiac troponin I in vitro. MSCs induced by VEGF-B expressed von Willebrand factor (vWF) and CD31 in vitro. MSCs implanted in myocardium were stained positively for cardiac myosin heavy chain and vWF. Conclusions MSCs can differentiate into myocardial cell and endothelial cell , are ideal seed cells in regeneration medicine.PART TWO Objective The preclinical study was designed to test the feasibility and safety of autologous bone marrow mesenchymal stem cell (MSC) transplantation by intracoronary injection in acute myocardial infarcted pig (AMI). Methods Coronary occlusion with balloon was used to produce AMI. Labeled MSCs were implanted by intracoronary injection. Four weeks after AMI, single photon emission computed tomography was used to evaluate the relative infarct size, myocardial perfusion score and ejection fraction (EF) of the animals. Left ventricular systolic pressure( LVSP), left end-diastolic pressure (LVEDP), peak rate of pressure rise(+dP/dt) and peak rate of pressure fall (-dP/dt) were assessed by catheterization. Immunofluorescence was performed to evaluate MSC engrafment and...