Oral Implant Soft Tissue Interface Bonding Mechanism And Improvement Of The Experimental Study Of The Factors

Endosseous oral implants must function in two seperate enviroments: the internal enviroment of bone and soft tissues and the external enviroment of the oral cavity which contains bacteria and debris. For the oral implant to survive in this dual enviroment, a viable biological seal that prevents the ingress of bacteria and other inflamation-producing agents must exist at the soft tissue-implant interface as well as a strong osseointegration at bone—implant interface. A greater understanding of the mechanisms of attachment and of the factors which enhence the integrity of the biologic seal between implant and soft tissues should permit an improved prognosis for functioning titanium implants.It has not been ascertained about the exact attachmentmechanisms of soft tissues to titanium implants. Difficulties in culturing purified oral mucosal basal cells and in preparing ultra- thin sections of the tissue/ implant interface are two obstacles to the research in this field. Usually, epithelial cells culture from periodontal ligment which are not match clinical situation are used in some of the researches. Few of ultrastructural studies of cell attachment to titanium implant refered the influences of titanium surface characters on their experimental outcomes. In this report, a series studies about the culture of oralmucosal basal cells, preperation of titanium film, ultrastructural studies of epithelial and fibroblast cell attachments to titanium film and the attachment improving effect on epithelial cell to titanium with basement membrane matrix Matrigel were carried out.This research includes four parts. Firstly, a model of culturing normal...