1.The Primary Culture Of Human Gingival Fibroblast Cells And Human Gingival Epithelial Cells2.Therapeutical Effect Of Guided Tissue Regeneration With Bio-Gide And Bio-Oss In The Treatment Of Periodontal Bone Defects

Objective:To establish and evaluate the primary culture methods of human gingival fibroblast (HGF), to establish an experimental model for studying in vitro and to investigate their biological features.And to detect the expression of protease-activated receptor (PARs) in HGF.Methods:The primary cells of HGF were cultured with enzymatic digestion—tissue explants method.The Cells were identified by means of morphological analysis and immunofluorescence. RNA isolation and the mRNA expression of PARs in HGF studied by reverse transcription-polymerase chain reaction.Results:The cultured cells were spindle-shaped, and had a positive reaction to antibodies against vimentin and a negative reaction to antibodies against keratin, Their growth of HGF obtained by the methods were satisfactory.HGF expressed PAR-1and-3receptors mRNA.but not expressed PAR-2and-4receptors mRNA.Conclusion:The cell lines established in this study are proved to be human gingival fibroblasts cells. The method is simple and feasible.The satisfactory growth condition of the cells justified their use in vitro for future research of periodontitis. HGF expressed PAR-1and-3receptors Objective:To observe the the therapeutical effect of guided tissue regeneration (GTR) with Bio-Gide and Bio-oss in the treatment of periodontal bone defects, and to evaluate the clinical efficacy, in order to find an effective treatment.Methods:5typical cases of bone defects resulting from periodontal diseases. When periodontal condition was improved after non-surgical therapy, then treated by GTR with Bio-Gide and Bio-oss. Periodontal probing depth (PD), clinical attachment level (CAL) and X-ray were reexamined and recorded before and several months after surgery and analyzed. Analysis were evaluated before and several months after treatment; to access the efficacy of patients.Results:six months after the surgery, both PD and CAL were significantly reduced. X ray showed that the low-density image area of the original defect was reduced, and bone density was significantly increased.Conclusions:GTR with combination of Bio-Gide and Bio-oss can get a stability of clinical attachment level gains in the treatment of periodontal bone defects.