Phthalates Toxic Effects On Drosophila

[Objective] To study the effect of phthalates including DEHP and DBP-DOP on the lifespan, anti-oxydative system, reproductive system, genotoxicity, and neuotoxicity of Drosophila melanogaste. [Methods] â‘  Effect of DEHP and DBP-DOP on the lifespan of drosophila. was studied by the longevity test â‘¡Determine the activity of total-SOD and CuZn-SOD and the concentration of MDA of the drosophila fed by DEHP orally, thus to study its impact on the lipid peroxidation in vivo.. â‘¢Use subacute toxicity test to study the damage of DEHP on the reproductive system of drosophila. â‘£To determine the effect of DEHP on differential gene expression of the brain tissue by the use of RT-PCR (gene chips) thus to study its toxicity on drosophila. ⑤The genotoxicity of DBP-DOP on the germ cells of drosophila. was studied by The sex-linked recessive lethal (SLRL) test The result of sex-linked recessive lethal (SLRL) test shows genotoxicity to reproductive cells in drosophila induced with DBP-DOP. [Results] â‘ 200mg/L dosage of DEHP and DBP-DOP shortened respectively the half lethal time (LT₅₀) of drosophila by 12d with the female and 10d with the male all compared with control(p<0. 01 or 0. 05); a time-dependence relationship was shown. Also, mean and max of the lifespan shortened (p<0. 01) . So DEHP and DBP-DOP shortened respectively the lifespan of drosophila. â‘¡Activity of total-SOD and CuZn-SOD of drosophila fed by DEHP was weaker than control (p<0.01 or 0.05) , however, concentration of MDA increased (p<0. 01 or 0.05) , thus to imply that DEHP inhibited the activity of SOD, promoted reaction of lipid peroxidation in vivo. â‘¢DEHP and DBP caused respectively reversible damage to the reproductive system in drosophila. (4)35 fragments were induced by DEHP in the study of differential gene expression of brain tissue. 20 of them were upregulated,including CYP6a2, CYP6a8, CYP6wl, CYP4p1, Ugt36Bb, GST, Carboxylesterase, Glycine N-methyltransferase, Oxidoreductase, Est10, Jheh1, etc. with 8 new findings. 15 fragments were downregulated, including Mst57Da, Mst57Db, Acp95EF, Os-E, Os-C, Pbprp3, Pbprp1, a10, etc. with 7 new findings. Those genes involved in the functions of signal conduct, cell metabolism, growth, tissue differentiation and transcription factors with some for further comprehension.⑤ The result of sex-linked recessive lethal (SLRL) test shows genotoxicity to reproductive cells in drosophila induced with DBP-DOP and promote both rates of mutagen and sterility respectively. [Conclusion] DEHP, DBP and DOP have toxic effect on Drosophila melanogaster indicating potential hazards on human-being.