Phage Display Peptide Library Display Technology Applied Research In The Screening And Construction Of The Cd59 Gene Activity Sites In The Tumor Escape

Objective: To isolate targeted mimicry peptides that binding human CD59, the Ph.D-12phage display library was conducted on human CD59 and its McAb respectively. Further,to design the short-peptide clamp of CD59 against tumor escape, and to be expected topresent some theoretical basis on biological effect of target drug of tumour.Methods: The CHO cellls with high expression of CD59 and McAb of CD59 werescreened. The screened phage monoclones were conducted specific binding efficiency withthe wild type and sequenced. The short-peptide clamp was designed on attained sequencewith high occurrence frequency, and inspected via transmission electron microscope (TEM)and immunohistochemistry.Results: The displayed phages have high recovery and specificity. The obtained 3sequences with high homology showed certain homology with sequence of human CD2(HXAXXXXXXPXX). By TEM and immunohistochemistry, the short-peptide clampshowed obvious depressant effect on tumour apoptosis inhibitory of CD59.Conclusions: The CHO cellls with high expression of CD59 and McAb of CD59through Ph.D-12 phage display library can obtain phage peptides with highly specificbinging ability. The short-peptides designed on the obtained one, showed obviousdepressant effect on tumour apoptosis inhibitory of CD59 and layed some theoretical basison biological effect of target drug of tumour.