Analysis Of Different Proteins Expression Between Papillary Thyroid Carcinoma And Normal Thyroid Tissue

Objective: In order to better understand the basic mechanisms of tumor development and identify potential new biomarkers of papillary thyroid carcinoma, the different proteins expression were investigated between papillary thyroid carcinoma and normal thyroid tissue.Methods: The total proteins of papillary thyroid carcinoma and normal thyroid tissue were separated by immobilized pH gradient (IPG)-based two-dimensional gel electrophorsis (2-DE), the 2-DE maps were established for papillary thyroid carcinoma and normal thyroid tissue respectively. The different expression protein spots were analyzed with Image Master 2D Elite5.0 analysis software, then the peptide mass fingerprinting of different expressed proteins were verified by MALDI-TOF-MS/MS. The functions of the different expressed proteins were identified through Swiss-port database searching.Results: VVe established the 2-DE maps of papillary thyroid carcinoma and normal thyroid tissue respectively. Sixty different proteln spots were found by image software and seventeen proteins were recognized with MALDI-TOF-MS. The biological information of these proteins was identified through Swiss-port database searching. Among the total, eleven overexpressed protei ns and six underexpressed proteins were found in papillary thyroid carcinoma The overexpressed proteins included cyclin-D2, Mdm2, Galectin-3, Cytokeratin 19, Cathepsin B, Manganese SOD, S100 A6, Moesin, Fer and so on. The underexpressed proteins were consisted of Peroxiredoxin-2, Hsp70, Hsp27, Protein Phosphatase 2, Vitamin D-binding and Actin cytoplasmic 1. Some overexpressed proteins were related to cell cycle control, cellular metabolism and invasion and metastasis of the tumor.Conclusion: Many different expressed proteins between papillary thyroid carcinoma and normal thyroid tissue were identified. These different expressed proteins perhaps have closed relation to the genesis and development of the papillary thyroid carcinoma through influence the cell cycle control, invasion and metastasis and cellar metabolism. Investigating these proteins is helpful to explore the basic mechanisms of tumor development and identify potential new biomarkers. We could benefit from early diagnosis of papillary thyroid carcinoma and development of target therapeutic agents.