Expression Of Pin1 In Non-Small Cell Lung Cancer

Lung cancer is the leading cause of cancer deaths in both womenand men throughout the world,and the incidence is still increasing.Today, no useful biomarker exists for detection of lung cancer.However, useof tumor marker for detection and treatment of lung cancer ispromising.Pinl is overexpressed in many different cancer types,includingbreast,prostate and ovarian carcinomas.This is the first report of Pinlexpression in clinical samples of Non-Small Cell Lung Cancer(NSCLC).Method: Protein expression levels of Pinl in 141 cases ofNon-Small Cell Lung Cancer(NSCLC) were analyzed for expression ofPinl using immunohistochemistry and compared to clinicopathologicalcharacteristics. The mRNA expression of Pinl in 60 cases of NSCLC wasalso analyzed using quantitative real-time RT-PCR and compared toclinicopathological characteristics.Results: 1. Immunohistochemical: Pinl protein was shown to be overexpressed in NSCLC samples. The expression of Pinl was correlatedwith lymph node metastasis and TNM stage. No significant correlationwas observed between Pinl levels and histology, differentiation, T stage,sex, age, family history and smoke condition. 2.RT PCR: High level PinlmRNA expression was significant correlated with the presence oflymph node metastasis and with the stage of the disease. There was also a significant correlation between the smoking history of a patient and thelevel of Pinl expression.Conclusion: Pinl was higher expressed in NSCLC and may be avaluable tumor marker for NSCLC.The expression of Pinl wassignificant correlated with the presence of lymph node metastasis andwith the stage of the disease.