| Objective: Tumor and the relationship between the body's immune system is very complicated. On the one hand, there is a cancer tumor surface antigen, the immune system can recognize such antigens and produce a series of immune response, leading to tumor rejection; On the other hand, some tumor immune deficiencies must be activated by the antigen molecules, or antigens has been attacked after the modulation, the tumor itself or the effect on immune function, so that the tumors evade the host immune to attack growth development. In the body, from a single tumor cells into cancer cells grow into cloned, and then a tumor, is a multi-step process. Tumor micro-environment, there are factors and immune suppression of tumor growth can produce and release excessive antigen, the immune cells have a direct and (or) the inhibition or even indirectly promote the role of his death. Cancer patient's immune and control the majority of inadequate or there are some flaws, and very complicated factors. Xianyu decoction is a mentor Professor Chen Ruishen from the pathogenesis of lung cancer, lung cancer balanced "sputum, weak, stasis" in three areas to strengthen splean, clean lung, eliminate phlegm and remove blood stasis for the treatment of law, since the experience to the treatment of lung cancer. Clinical application of the side for many years, confirmed that a better clinical effect. This issue through observation Xianyu decoction with IIIb and IV chemotherapy in the treatment of immune function in NSCLC, and cytokines from the area of its mechanisms for further research and development Xianyu decoction provide experimental basis.Methods:1. Clinical research: 90 patients with stage IIIb or IV NSCLC were divided randomly into XianYu Decoction group, chemotherapy group and combined group, the group of two courses (six weeks), compared with the combined group cents soup, in the chemotherapy group of natural killer cells (NK), T cell subsets such as the impact.2. Experimental studies: Lewis lung cancer cells were inculcated to 50 C57BL/6J mice vaccinated since Lewis lung cancer tumor cell lines after 24h, mice weighing, number, then divided into five groups, each with 10, Xianyu decoction, the Combined (treated with cisplatin and Xianyu decoction) group, chemotherapy (DDP) group, the control group, normal control group, Chinese medicine can stomach high-dose group, 2.2g/20g mice, each group are 0.2ml gavage, the chemotherapy group and the Joint Group to Low-dose cisplatin 0. 02mg/20g/d, dl—21, tumor model group and the control group to 0.2ml saline gavage. The mice were gavage for 21 days, using FQ-PCR detected lewis lung cancer tumor cells of mice immunosuppressive factor TGFβ1 mRNA, IL-10mRNA situation.Results: Clinical observations: (1)Xianyu decoction after treatment, NK cells, CD3~+,CD4~+,CD4~+/CD8~+than before treatment has improved, CD8~+ lower than before treatment, the difference was statistically significant (P <0.01, or P<0.05).(2) The Combined Group after treatment, NK cells, CD3~+,CD4~+,CD4~+/CD8~+increase, CD8~+ lower than before treatment, the difference was statistically significant (P<0.01, or P<0.05).(3) After treatment with chemotherapy group compared to before treatment, NK cells, CD3~+,CD4~+ lower, CD8~+ increase than before treatment, CD4~+/CD8~+ lower than before treatment, the difference was statistically significant (P<0.01, or P<0.05).(4) Xianyu decoction Group and the Combined Group after treatment of NK cells, CD3~+,CD4~+,CD4~+/CD8~+increase, CD8~+ lower, with the chemotherapy group, the difference was statistically significant (P <0.01).Experimental studies:(1)The Combined Group and Xianyu decoction, chemotherapy group, model group,the Combined Group Lewis lung cancer can reduce the TGFβ1 mRNA expression,the difference was significant (P<0. 01).(2) The Combined Group and Xianyu decoction Group, chemotherapy group, modelgroup, the Combined Group Lewis lung cancer can reduce the IL-10mRNA expression,the difference was significant (P<0.01).Conclusions: Clinical research suggested Xianyu decoction Group, Xianyudecoction with chemotherapy cents to improve IIIb and IV non-small cell lung cancer patients with immune function, and Xianyu decoction alone cents a better effect. Some of radiotherapy and chemotherapy toxicity can not be tolerated or elderly patients, if necessary, can be used alone cents soup treatment, by enhancing immune function in patients with cancer and confrontation, Xianyu decoction and cents alone had no significant side effects, can further reduce the suffering of patients and improve survival Quality.Experimental study suggested Xianyu decoction with low-dose chemotherapy, Lewis lung cancer can reduce the immunosuppressive cytokine TGFβ1 mRNA, IL-10mRNA expression, suggesting that this may be treated cents soup IIIb and IV NSCLC Phase one possible mechanism.
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