The Investigation On The Induced Difdrentiation And The Proliferation Of Human Hepatocacinoma SMMC-7721 Cells By Oldenlandia Diffusa

OBJECTIVE:To induce cancer cells to differentiate into normal cells is a new branch of Oncotherapy,and has become more and more concerned in this field.Presently,the common differentiation-inducing agents still have various side effects,while lack high efficiency of induction. Additionally,there has been lots of investigation on non-solid tumor,but not satisfying.Thus,it is a key tosearch for non-toxic and natural origin substances that can induce the differentiation of cancer cells efficiently,especially for the therapy of solid tumor,which needs futher studies and researchs to resolve.It is reported that Oldenlandia diffusa can inhibit many kinds of tumor cells.However,it remains unclear that Oldenlandia diffusa can induce differentiation of,solid tumor,such as hepatocarcinoma cells.In this study,the differentiation-inducing effects of Oldenlandia diffusa on SMMC-7721 hepatocarcinoma cells and the differentiation mechanism were investigated.This study may provide helpful theoretical and experimental envidence to develop new methods for hepatocarcinoma therapy.METHODS:The effects of Oldenlandia diffusa were investigated in vitro,through culture SMMC-7721 with in 20%OD serum contain Oldenlandia diffusa,by using light microscope,electronic microscope,immunohistochemistry,Flow Cytometer.Then we measured growth curves of SMMC-7721.cells,cell proliferation index,cell inhibition ratio,cell cycle,the rate of colony forming in SMMC-7721 heptocarcinoma cells,the microstructure of cells,the expression level of Alpha fetoprotein mtp53,bcl-2,CyclinD,and Ki-67 to evaluate the differentiation-inducing effects of Oldenlandia diffusa on SMMC-7721 heptocarcinoma cells.RESULTS:Oldenlandia diffusa inhibited obviously the proliferation of SMMC-7721 heptocarcinoma cells,deceased the rate of colony forming in cancer cells,which were made to stay in G1 stage.Furthmore, Oldenlandia diffusa significantly deduced the expression level of Alpha fetoprotein mtp53,bcl-2,CyclinD,and Ki-67.CONCLUSIONS:Oldenlandia diffusa has the effects of inhibiting the proliferation and growth,promoting differentiation and apoptosis on SMMC-7721 hepatocarcinoma cells.Oldenlandia diffusa maybe a potential agent for differentiation-induction of SMMC-7721 hepatocarcinoma cells.