The Influence On Metabolic Remodeling β3 Receptor And APPRα Of HF Rat Model Treated By Xinmaitong

Objective:To observe myocardial cell chondriosome energy metabolism Reconstitution and hemodynamic diversity in HF rat model,the effect on FFA, LD,SDH,ATP enzyme content in musculature,β₃ receptor and PPARαexpression,and to approach the mechanism of Xin Mai Tong treating HF.Methods:75 rats were divided into five groups by random digits table: blank group,model group,Xin Mat Tong group,Carvedilol group and Fenofibrate group.We set up model with Adriamycin,and interfered with Xin Mat Tong,Carvedilol,Fenofibrate respectively.Then we recorded hemodynamic diversity by Electro-physiograph,determined changes of FFA,LD, SDH,ATP enzyme content with tissue bomogenate method,detectedβ₃ receptor and PPARαexpression in musculature by immunohistochemistric method and RT-PCR,and observed the effect of myocardial cell,β₃ receptor and PPARαtreated by serum that contained Xin Mai Tong with serum pharmacology method.Results:1.HR,+dp/dtmax and modulus of dp/dtmin in model groups all showed lower than blank group,and the comparison had significant deviation in statistics(p＜0.05,p＜0.01,p＜0.01),but LVEDP showed higher than blank group, so there was significant deviation in the comparison(p＜0.01).HR,+dp/dtmax and modulus of dp/dtmin in Carvedilol group and Xin Mai Tong group were higher than model group,while LVEDP lowed down,and between these comparisons all expressed statistical significance(p＜0.05).The others showed no significant deviation(p＞0.05).Fenofibrate group didn't improve rats' hemodynamics parameters,so the results had no statistical significance (p＞0.05).2.The comparison of BM/ VM between Xin Mai Tong group and model group indicated significant deviation(p＜0.01),but the comparisons in Carvedilol group,Fenofibrate group and model group didn't have the result (p＞0.05). 3.FA and LD levels in rats' musculature were obviously lower than model group,which showed significant deviation(p＜0.05).LD levels in Xin Mat Tong group and Fenofibrate group were lower than Carvedilol group(p＜0.05).Both of Carvedilol and Fenofibrate couldn't change SDH activity,but it changed in Xin Mai Tong group,and these comparisons showed discrepancy through statistical treatment.Na⁺K⁺-ATP and Ca²⁺Mg²⁺-ATP activity in Xin Mai Tong group and Fenofibrate group were obviously improved(p＜0.05),Carvedilol also increased these two enzymes activity,but it had no statistical significance (p＞0.05).4.PPARαin musculature expressed lower in model group than in blank group,and it expressed higher in Xin Mai Tong group,Carvedilol group and Fenofibrate group in model group,but we couldn't find obvious difference in the three groups under microscope.β₃ receptor expressions all showed higher in model groups than blank group,it expressed a bit higher in Fenofibrate group than Carvedilol group,its expression in Xin Mai Tong group reduced comparing with Fenofibrate group and Carvedilol group.5.β₃ receptor expressed higher in model groups than in blank group,which showed significant deviation(p＜0.01),comparisons in Xin Mai Tong group, Carvedilol group and Fenofibrate group also indicated significant deviation.β₃ receptor mRNA expression obviously reduced(p＜0.01),and all lower than blank group(p＜0.01).It lowed down much more in Xin Mai Tong group than in Carvedilol group(p＞0.05),but compared with Fenofibrate group,there was no significant deviation.6.After H₂O₂ had effected 6 hours,cells vigour significantly reduced (p＜0.05).The cell vigour in Xin Mai Tong group was lower than blank group, but higher than model group,and the comparison indicated statistical deviation (p＜0.05).7.β₃ receptor expression rose in model group after H₂O₂ damaging 6 hours (p＜0.05),also the result in Xin Mai Tong group expressed similar(p＜0.05). PPARαexpressed lower in model group than in blank group(p＜0.05),but given serum with Xin Mai Tong,it increased(p＜0.05).Conclusions:1.Myocardium in HF rat model by Adriamycin showed metabolic reconstitution.2.Xin Mai Tong could improve cardiac function of HF rat model by Adriamycin,raise BMI and survival rate,and modify contents of FFA,LD,SDH, ATP enzyme.3.Xin Mai Tong could improve HF rat model metabolic reconstitution through reducingβ₃ receptor and increasing PPARαexpression.4.Serum with Xin Mai Tong could produce a marked effect on energy metabolism in myocardial cell chondriosome that induced by anti-H₂O₂,through inhibitingβ₃ receptor excessive activation and enhancing PPARαexpression.5.Xin Mai Tong could treat HF by improving metabolic reconstitution.