The Effects Of Soothing Liver And Activating Spleen Method On The Hypersensitive Of The Diarrhea-Predominant Irritable Bowel Syndrome And The Research Of This Mechanism

Aim:1,To study the effects of soothing liver and activating spleen method( on the hypersensitive of the diarrhea-predominant irritable bowel syndrome patients;2,.To study the mechanism of soothing liver and activating spleen method altered the the hypersensitive of the diarrhea-predominant irritable bowel syndrome patients;3,To study the effect of soothing liver and activating spleen method on the IBS model rats.Method: 1,50 patients with IBS whose TCM syndrome type is liver stagnancy with spleen deficient (LSSD) was divided into placebo group and intervention group through randomly double blind Case-Control method, each group has 25 patients , one group was treated with capsule Kangtai which TCM treat method is mainly soothing liver and activating spleen method , 3times one day ,4 capsules one time, course of treatment is about 4weeks;one group was treated with placebo capsule (the main component is starch),the 15 healthy subjects were served as controls. The rectal sensitivity was chected by Syectics Viceral Stimulator ;2,the serum 5-HT concentrationgs and the 5-HT concentrationgs in the the ileocecal mucosa and sigmoid mucosa was measured by high performance liquid chromatography and. fluorescence chromatography ;3,The number of mast cell in the the ileocecal mucosa and sigmoid mucosa was measured;4,Neonate rats were randomly divided into normal group, model group, capsule Kangtai group. In order to replicate the IBS model rat , The rats were. give 0. 5% acetic acid 0.5ml in rectum every day from postnatal 8d to21d. Then threshold abdominal withdrawal reflex (AWR ) of rats was measured at 6w mid 8w with rectal distension.Result: 1,Compare to the controls there is no significant difference in the rectal Resting pressure,the resting pressure and squeeze pressure of anal sphincter and sphincter (IASMSP) from placebo group and intervention group before and after treating. before treating rectal maximum tolerable volume threshold and defecation volume of placebo group and intervention group is significantly lower than the contol (P<0.05). After treating the rectal maximum tolerable volume threshold and defecation volume of intervention group is increased ,there was no significant difference with the control group(p>0.05) .2,before treating the serum 5-HT concentrationgs and the 5-HT concentrationgs in the ileocecal mucosa and sigmoid mucosa is significantly higher than the contol (P<0.01) After treating the serum 5-HT concentrationgs and the 5-HT concentrateiongs in the the ileocecal mucosa and sigmoid mucosa wasdecreasedin intervention group. there was no significant difference with the control group (p>0. 05) ,. the 5-HT concentrationgs in the the ileocecal mucosa is little higher than sigmoid mucosa, but there is no significant difference. 3,Before treating the number of mast cell in the the ileocecal mucosa of intervention group is 10. 57±1.66, It is higher than the controls 5. 67±1. 06(P<0. 01) ;After treating the number of mast cell in the the ileocecal mucosa of intervention group is 5.93±0.89, there was no significant difference with the control. Before and after treating the number of mast cell in the ileocecal mucosa of placebo group is 11. 27±2. 61and 9. 83±2. 52, but there was significant difference .The number of mast cell in the sigmoid mucosa of placebo group and intervention group has no significant difference .with the conrol.4,The threshold of AWR in model group, capsule Kangtai group. was obviously de creased than that of model control group. After 2weeks treating The threshold of AWR capsule Kangtai group is obviously increased(P<0.01) .there was no significant difference with normal group.Conclusion:1,soothing liver and activating spleen method can decrease the hypersensitive of the diarrhea-predominant irritable bowel syndrome;2,there is some relationships between hypersensitive of the diarrhea-predominant irritable bowel syndrome with liver stagnancy with spleen deficient (LSSD) ;3,there is some relationships between hypersensitive of the diarrhea—predominant irritable bowel syndrome with 5-HT and MC, soothing liver and activating spleen method can decrease the hypersensitive of the diarrhea-predominant irritable bowel syndrome by effecting the 5-HT signal pathway.