Human Papillomavirus Genotyping And Its Clinical Significance

Cervical cancer,a common gynecologic malignancy,is a serious threat to women's health in china.Epidemiological studies have shown that the persistent infection of high-risk HPV is the most important factor for cervical cancer formation. The effective way to decrease the cervical cancer morbidity is to detect and classify the high-risk HPV infection in early time.In this study,we have established a rapid high-throughput HPV genotyping method by using the suspension array technology(HPV suspension array,HPV-SA). The specificity had been confirmed by the hybridization between probes and standard samples.Experiments show that HPV-SA can quickly and accurately detect 720 clinical samples.The detection accuracy of HPV using HPV-SA for 720 samples was as follows:sensitivity 86.14%,specificity 98.26%,positive predictive value 95.08%,the negative predictive value 92.92%,consistency 94.86%.In order to obtain subtypes information of HPV infection in the region,as well as understand the relationships between HPV subtypes and cervical lesions.We have studied on the HPV prevalence in different cervical lesions by using the HPV-SA method.Experiments show that the major prevalent high-risk HPV subtypes were HPV16,58 and 18 in normal,CINâ… ,CINâ…¡and CINâ…¢tissues,while in carcinoma tissue were HPV16 and 18.The single infection rate was significantly higher than multiple infections in all lesions.This information can improve the effect of cervical cancer screening,as well as provide important information for HPV vaccine design.