Tune The Experimental And Clinical Studies Of The Liver The Spleen Of The Intervention Effect Of Alcoholic Liver Fibrosis

Alcoholic liver fibrosis(ALF) is the compensated reaction in the tissue repair of inflammation or injury of liver after drinked long time and extracellular matrix(ECM) excess deposited in the liver is its pathological feature. Alcoholic liver disease is the main and first cause of the liver cirrhosis in the western countries and the second in China. The main forming mechanisms of Alcoholic liver fibrosis is the activation of hepatic stellate cells(HSC) which turn into fibroblast-like cell with the changing of phenotypes and function to keep and aggravate ALF through making of much extracellular matrix by autocrine and paracrine secretion. The western medicine still has not the curative drug of ALF, but Traditional Chinese Medicine has the sure clinic curative effect and dominance by the integral therapy theory and determination of treatment based in pathogenesis obtained through differentiation of symptoms and signs.Purpose:To research the therapeutic action of Tiaoganlipi Decoction to ALF and to interpret the mechanisms of action by clinic research trial and animal experiment.The paper is composed of three parts:the review articles study, the clinical research and the experimental study.The review articles studyThere are two parts in the theoretical study, one summarized the cognition of the name, cause, mechanisms and treat of alcoholic liver fibrosis by the theory of TCM, the other discussed the research production of modern medicine about mechanisms of alcoholic liver fibrosis.The clinical researchApproachTaking the Tiaoganlipi Decoction as the treatment group and the Fufangbiejiaruangan Tablet as the control group by the random and parallel control study at the base of former research, the 86 cases of ALF were involved during this period of the trial, and among them the 43 cases were in treatment group, 43 cases were in control group. After 4 weeks treated,according to the difference of clinical symptom, signs and the result of biochemistry testing, serum testing of fibrosis, B type ultrasonography of pretherapy and post-treatment, it was to be compared by the curative effect assessment standard to deepen the clinic research of the Tiaoganlipi Decoction.Result: The total accumulated symptoms integral of the two groups all had decreased significantly after treated and there was significant difference(P<0.05). The total effective percentage of the treatment group was 90.7%,of the control group 74.4%. There were significant differences between the two groups (P<0.05). The effect of treatment group was more advanced than of the control group for relieving the syndrome of diarrhea and acratia and had significant difference(P<0.05). Both the two groups could decrease the content of ALT,AST,PIIINP and HA(P<0.05),but having no significant differences between the two group(sP>0.05). Both the two groups could decrease the content of GGT,CGIV and LN(P<0.05), and the effect of treatment group was superior to the one of the control group(P<0.05).Conclusion1Tiaoganlipi Decoction can improve the symptoms, liver function and liver fibrosis'testing result of ALF and has no obvious adverse effect. So it is a safe and effective compound preparation.2 On the whole, Tiaoganlipi Decoction and Fufangbiejiaruangan Tablet all had the curative effect to the stagnation of liver-QI with deficiency of the spleen, stagnation of blood and phlegm of ALF, and the curative effect of Tiaoganlipi Decoction was better than the curative effect of Fufangbiejiaruangan Tablet.The experimental studyApproachIt involved in the vivo experiment. Firstly, the male mice of species of Kunming were experimented to select the best abstraction technology of Tiaoganlipi Decoction, in which is the second abstraction technology. And then to make the rats model of ALF by intragastrical method with the 50% alcohol, simultaneously the intervention is to be given. By testing the AST, ALT, HA, LN, CIV in the serum and SOD, MDA, HYP in the liver tissue, CYP2E1 in liver tissue by RT-PCR testing,α-SMA in liver by immune tissue chemistry testing and the HE and Masson dyeing testing the liver tissue, to research the mechanism of action of Tiaoganlipi Decoction to resist the hepatic fibrosis of ALF.Result1 The mechanism of action of Tiaoganlipi Decoction to resist the hepatic fibrosis of ALF: induced the body to produce much antioxadation factors, decreased the expression of the Cytochrome P4502E1 in liver, raised the clearing ability of free radicle to pertect the liver cell and to cut down the pathological high transaminase, to cut down the CIV and HA in the serum of ALF rats. So it can prevent the process of ALF and interrupt the proceeding of ALF by decreased theα-SMA.2 The treatment group of Tiaoganlipi Decoction and the control group of Fufangbiejiaruangan Tablet all can improve the liver function and regress the hepatic fibrosis. Totally, the curative effect of Tiaoganlipi Decoction is better than Fufangbiejiaruangan Tablet.Conclusion1 Tiaoganlipi Decoction can effectively prevent and regress the generation and development of ALF, it had the good curative effect in the resisting the ALF.2 The mechanism of action of Tiaoganlipi Decoction to regress the ALF is that it resisted the oxygen free radical, decreased the expression of the Cytochrome P4502E1 andα-SMA in liver and interrupt the several pathological joint of hepatic fibrosis.