Infuluence Of Electroacupuncture On Non-Alcoholic Fatty Liver Cytochrome P4502E1 Expression And Owidation's Impact

Objective:To investigate the infuluence of electroacupuncture on non-alcoholic fatty liver cytochrome P450 2E1 (CYP 2E1) expression and oxidation's impact. To investigate the mechanism by which cupuncture treats nonalcoholic fatty liver rats.Methods:Thirty-three male SD rats were randomized into 2 groups : normal control group﹙n=11,fed with normal food﹚ and NAFLD rats﹙n=23,fed with high-fat food. Eight weeks later 1 rats were randomly selected from the normal control group and 2 rats were randomly selected from the NAFLD group and were killed to under go pathological examination of the liver. When the establishment of experimental model of NAFLD rats was confirmed the remaining 20 NAFLD rats were sublishment into 2 equal subgroups: NAFLD control group﹙ to be fed continuously with high-fat food﹚ , acupuncture treatment group﹙to fed continuously with high-fat food﹚. Take "Zusanli", "Hong Leong," "Sanyinjiao", " Taichong" Point, electroacupuncture - spacing parameters, 2Hz frequency, intensity 4mV to the appropriate vibrating slightly lower limb.By the end of the 12th week, Group 3 rats were fasted for 24 h, weighing, ether anesthesia, the heart puncture blood, a blood cleansing in the plastic tube in the static, low-temperature centrifuge separation in the serum (3000 r / min, 10min), home -20℃cryogenic refrigerator preserve analyte. Measurement of serum free fatty acids (FFA), the animals were sacrificed, open abdominal cavity, and then quickly removed the liver, said wet weight, detection of liver index, each of the left hepatic are based on three, respectively with 10% formalin-fixed, paraffin - Buried for the pathology section;The remaining liver saline rinse, Filter Shian, said from 1 g, by adding 10 ml saline ice in the ice water in the homogenate. Made 10 percent homogenate. 3000 rpm 10 min centrifugal supernatant from detection intrahepatic malondialdehyde (MDA), superoxide dismutase (SOD) , triglycerides (TG) , total cholesterol (TC) , glutathione (GSH) content changes. HE staining of liver biopsy pathological changes in their patterns, immunohistochemical detection of hepatic cytochrome CYP 2E1 expression using immunohistochemical results IPP5.1 immunohisto-chemical analysis software, each biopsy were randomly selected five typi-cal color vision, for each accumulated a specific vision optical density value (intergrated optical density, IOD), a select few of the sections are the coloring strength, the results of x±s said. Statistically using analysis of variance and q test. Result :1.eight weeks after the end of Model liver istopathological examination:Optical microscopy normal rat liver cells are closely into cable-only individual cells with a small amount of lipid droplets, the model rat liver cells of a large number of fat-like vacuoles were transparent, avitation nuclei were fat Liver cells hold he brink of histologic examination proved modeling success.2.12 weekend killed three rats, the results showed that: Electroacu-puncture group compared with the model group, serum FFA levels, body weight, liver index decreased significantly (P <0.05), liver TC, TG, MDA was significantly lower (P <0.01), liver SOD , GSH was significantly higher (P <0.01). Electroacupuncture serum FFA levels, body weight, liver index decreased value higher than the normal group (P <0.05), liver TC, TG, MDA value higher than the normal group (P <0.01), liver SOD, GSH value of the control group ( P <0.01).Histopathological observations, the model group are all animals in mo-derate to severe fatty degeneration of the liver cells, liver cells massive swelling edema, lipid droplets in the cytoplasm and more as a bubble-type, even a bit necrosis , the District of inflammatory cell invasion Run Obviously, acinar zone 3 fatty change than 2,1-district, electroacupuncture group of non-alcoholic fatty liver lobule and restoring, liver cells of the cable clearer, liver cells only a few small vacuoles fat , hepatic steatosis significantly reduced.CYP2E1 immunohistochemical results show that normal hepatic CYP2E1 expression in the central venous around several cell layer, no more than acinar zone 3. Model group showed diffuse hepatic CYP2E1 expression in the central vein to the surrounding area is obvious acinar 2,3 extended to an area. Electroacupuncture group hepatic CYP2E1 expression in the central venous acinar around to the three districts for significantly extended to two areas, one area did not see the expression (see picture).Compared with model group, electro-acupuncture group CYP2E1 IOD values decreased significantly , group differences were statistically significant (P <0.01) compared with the normal group, the electroacupunc-ture group CYP2E1 IOD value increased , group differences were statistically significant (P <0.01), compared with the normal group, model group CYP2E1 IOD values were significantly higher , group differences were statistically significant (P <0.01).Conclusions:1. electro-acupuncture therapy effect on NAFLD rats2. levels of the FFA for NAFLD in blood, body weight, liver index, liver MDA, TG, TC significantly reduce the role of rat hepatic GSH NAFLD, SOD has significantly increased role. 3.electro-acupuncture therapy can significantly inhibit NAFLD rat hepatic CYP2E1 expression Histopathological examination electroacu-puncture group fatty liver degeneration and inflammatory injury can be significantly improved.4.The above results confirm that electroacupuncture treatment in-hibited the expression of hepatic CYP2E1 , reduce lipid peroxidationof non-alcoholic fatty liver was obvious effect. Although the mechanism is too clear, but what could be electro-acupuncture treatment significantly decreased serum FFA concentration, CYP2E1 enzyme activity decreased significantly, inhibition of hepatic CYP2E1 expression, reduce lipid peroxidation, fatty liver and the degree of inflammatory changes Damage can be improved.