To Construct The Tissue Engineering Stent Model Of Knee Joint's Cartilage In Vitro And To Culture Cartilage In Vivo

Background: Because of its structure and features of articular cartilage of the knee operation in the highest incidence. Articular cartilage of the extremely limited capacity for self-repair. Traditional treatment methods including joint lavage and debridement in the joints, cartilage bone drilling, cutting flour type of cartilage, micro-fracture of. The mechanism of these technical repair articular cartilage are excited to achieve the intrinsic reaction of articular cartilage repair. Since the restoration through fibrous cartilage rather than hyaline cartilage, and can not be restored to the normal structure of cartilage damage, have taken place in the final degeneration. The treatment strategy is the application of tissue transplantation, including periosteal membrane and cartilage transplantation, cartilage or bone graft and cartilage cell transplantation. These methods of improving joint function and alleviate symptoms in varying degrees role. But they have different applications such as restrictions immune rejection reaction, the spread of infectious diseases, the impact of age, cartilage defect size preferences. With the speed of sound development of tissue engineering, makes soft tissue engineering for the repair of articular cartilage injury opened up a new way. Lots of documents proves that using cartilage tissue engineering, can have a similar normal articular cartilage in hyaline cartilage organizations. Practice shows that the use of tissue engineering technology, is expected to reach injury reconstruction objective, namely, to produce and transparent articular cartilage function and the same biomechanical properties of the organization.Research on cartilage tissue engineering has three basic elements: seed cells, frame, Construction Technology Organization. The purpose of this project is to bone marrow stromal cells cultured in vitro short passages, and amplified composite vector DLLA after implantation nude mice, using in vivo micro-environment and culture are basically the same as normal cartilage organizations, and the use of reverse vector DLLA engineering, CAD techniques and the construction of a cartilage lesion matching modelMethods: This study anatomy from the perspective of the people of knee cartilage morphology analysis, the subsequent use of spiral CT angiography after knee scan measurements to obtain basic theoretical data, a three-dimensional model, CTA scan as a data source using reverse engineering principles, and rapid prototyping technology to create the necessary repair of cartilage defects of the three-dimensional scaffold model. At the same time short-term cartilage cells cultured in vitro, amplified, and after the passage of the former, nude mice implanted training, and finally made basically the same as normal cartilage organization.Results: The cartilage cell differentiation is extremely weak, the ultimate traditional cultured cells to divide, and so there are many problems. Articular cartilage at the same time in different individuals and different parts morphological difference larger joints, cartilage cells passages, as well as amplification of the individual stent manufacturing is necessary.Chondrocytes in vitro and in vivo as well as training on CTA data, the reverse engineering of the individual stent is very necessary. These two technologies can be fully integrated to a greater extent meet clinical requirements cartilage defect for the future of cartilage repair has created new possibilities.