Clinical And Experimental Research On Gubiqing-Recipe In The Treatment Of Knee Osteoarthritis Complicated With Gonarthromeningitis

In this study,we take the medical theory of Traditional Chinese medicine (TCM) as a guide and investigate the mechanism of Gubiqing-Recipe in knee osteoarthritis (KOA) complicated with gonarthromeningitis from the theoretical study, clinical observations and experimental study.KOA is induced by damp-heat toxin for the Arthralgia Syndrome. Arthralgia Syndrome is caused by damp-heat, phlegm and blood stasis which are coagulated and accumulated into toxin, blockage of qi-blood circulation and damages of bones joints. We advocate using clearing away the heat-evil, expelling superficial evils, removing dampness as the chief treatment, and activating blood circulation to dissipate blood stasis, strengthening the body resistance meanwhile. The patients were treated with Gubiqing-Recipe that on establishment of tutor years of clinical experience of KOA. To explore possible action mechanism of KOA treatment of clinical efficacy from the macro and the cellular level from micro. 98 cases with KOA complicated with gonarthro- meningitis were randomly divided into test group (Gubiqing-Recipe) and control group (Xinhuang Tablet). Two groups take the indicators for observation such as total curative effect, TCM syndrome, WOMAC questionnaire, the clinical symptoms and signs of a single overall score and score symptoms and signs. Clinical efficacy and safety in accordance with time-phased medication was evaluated. The results show that Gubiqing-Recipe can significantly improve the KOA complicated with gonarthro- meningitis patients'TCM systemic symptoms, physiological functions,symptoms and signs. The Gubiqing-Recipe group curative effect surpasses the Xinhuang Tablet group. In observation period curative effect superiority along with treatment time expands. Gubiqing-Recipe has not obvious poisonous side reaction and has highly the security. We raise the OA-FLS cells in vitro of KOA complicated with gonarthromeningitis patients'synovial membrane, and observe the influence of different concentrations Gubiqing-Recipe and Xinhuang Tablet on cell growth and proliferation by MTT, flow cytometry and RT-PCR methods. The results show that Gubiqing-Recipe and Xinhuang Tablet can suppress the OA-FLS multiplication. Two medicines may suppress the OA-FLS mitosis in the physiological dosage, reduces the S time, lengthens the G1 time and the highly concentrated Gubiqing-Recipe can induce the OA-FLS cell apoptosis. Gubiqing-Recipe can obviously inhibited c-fos and COX-2 mRNA expressions of OA-FLS. Conclusion: Gubiqing-Recipe of clearing heat, detoxication, dispelling damp can inhibit OA-FLS cells'proliferation and differentiation directly from the transcription level. Gubiqing-Recipe display conformity control action through multi-ways, multi-links and multi-target. Gubiqing-Recipe can reverse basic pathogenesis that blockage of hot, damp, stasis and poisonous, restrain inflammation of synovium , decrease cartilage destruction, yield a radical cure of KOA complicated with gonarthromeningitis.