The Study Of Molecular Mechanism Of Nasal And Paranasal Sinus Malignant Tumor And The Micro Anatomic Structure Of The Orbit

It's very important to research the anatomy of nasocular correlation and malignant tumor in nose.There is no large amount of report for this aspect.There are a large amount of clinical cases in the study on the level of molecules for multiple genes,etiology,prognosis,and outcome in nasal tumors.There are 5 parts in study. There are important parameters in clinical significance by results from statistical analysis.Part 1Objective:(1) To study the micro anatomic structure of the nasocular correlation;(2) To discuss the distribution of the micrangium and micro lymph vessel;(3) To provid important anatomic parameter for the clinical diagnosis,treatment and prognosis of surgery about the nasocular correlation.Methods:The HE and the immunohistchemical technique ware used to detected the fossa orbitalis paries of the rabbit and human.Results:(1) The 13%ethylene diamine tetracetic acid and microwave(EDTA-MW) is the rapiddest decalcification technique and the best staining of the HE and immunohistochemistry;(2) There are four orbital walls on rabbit orbit:paries superior orbitae,paries anterior orbitae,paries inferior orbitae,paries posterior orbitae.The maxillary sinus is the well developed sinus in the rabbit.The paries anterior orbitae is the location of the nasocular correlation;(3) The CD34 and D2-40 protein was expressed in structure of the nasocular correlation in the rabbit,and the positive rate of the CD34 is highter than the D2-40. Conclusion:(1) The 13%EDTA-MW is the first selection of the decalcification techniques;(2) There is a complete fossa orbitalis in rabbit head.The rabbit can be an animal model on studying the disease between the fossa orbitalis and the nasocular correlation;(3) There are redundant blood vessel and small amounts lymph vessel in the correlated structure of nose and eye.Part 2Objective:(1) To study the epidemiological feature and pathogenetic regularity of the nasal and paranasal sinus malignant tumor(NPMT);(2) To provid the important parameter for the clinical diagnosis,treatment and prognosis of the NPMT.Methods:137 patients with NPMT were reviewed retrospectively.Results and Conclusion:(1) Age of onset of the NPMT is constrated between 40 and 76 years;(2) There are many pathological types in MPMT,the disease incidence of the epithelial tissue tumor is higher than the soft connective tissue tumor,the disease incidence of squamous carcinoma is the highest in NPMT;(3) The MPMT encroach many location easily,such as other paranasal sinus,fossa orbitalis,and intracalvarium.The MPMT has complicated clinical symptom;(4) The inverted papillorna and rhabdomyosarcoma of the nasal cavity and paranasal sinus can encroach the circum-structure easily,but the malignant melanoma and malignant lymphoma is contradictory. Part 3Objective:(1) To study the clinical significance of the p53,p63,p73 and nm23H1 genes in NPMT,and discuss the molecular mechanism of the genesis and development in the NPMT;(2) To provid the important parameter for the etiological factor,diagnosis, treatment and prognosis in the NPMT.Methods:The immunohistchemical technique was used to detected the expression of p53,p63,p73 and nm23H1 inNPMT.Results:(1) The positive rate of the p53 and nm23-H1 in NPMT was significantly higher than para-cancer tissues and non-cancer tissues(P＜0.01);(2) There was a significance on the expression of the p53 and p63 in endepidermis and in soft connective tissue of the NPMT(P＜0.01);(3) The expression of p63 and p73 was no significance among the NPMT, para-cancer tissues and non-cancer tissues(P＞0.05);(4) There wasn't statistical significance on the expression of the p73 and nm23H1 both of endepidermis and in soft connective tissue in the NPMT(P＞0.05).The expression of p73 was not relative to the nasal and paranasal malignant melanoma nor esthesioneuroblastoma(P＞0.05).(5) There was not significance on the expression of the p53,p63,p73 and nm23-H1 in different encroachment degree of the NPMT(P＞0.05)Conclusion:(1) The p53 and nm23-H1 perhaps participate in regulation of occurrenc in NPMT;(2) The p53 and p63 maybe play different role in different pathotype of the NPMT;(3) The expression of p73 may not be associated with tumorgenesis of NPMT.The p53,p63,p73 and nm23-H1 may not be associated with tumorgenesis of NPMT. Part 4Objective:(1) To study the invasion and metastatisis of the NPMT;(2) To provid the important parameter for the etiological factor,diagnosis, treatment and prognosis of the NPMT.Methods:The immunohistchemical technique was used to detected the expression of E-Cad,LN,ColⅣand LN-R in NPMT.Results:(1) There was a significance on the expression of the E-cad,LN,LN-R and ColⅣin NPMT,para-cancer tissues and non-cancer tissues(P＜0.01);and the positive rate of the LN-R in nasal inverting papilloma is significantly higher than that of nasal polyp and cystis(P＜0.01);(2) There was a significance on the expression of the E-cad and LN-R in endepidermis and soft connective tissue of the NPMT(P＜0.01);the expression between LN and ColⅣwas no significance(P＞0.05);(3) There was a significance on the expression of the LN-R and LN in different encroachment degree of the NPMT(P＜0.05);the expression of the E-cad and ColⅣwas not significant(P＞0.05).Conclusion:(1) The E-cad,LN,LN-R and ColⅣperhaps participate in regulation of occurrenc in NPMT,and LN-R perhaps participate in regulation of occurrenc in nasal inverting papilloma;(2) The E-cad and LN-R maybe play different role in different pathotype of the NPMT,but the LN and ColⅣmay be play the homoioplastic role:(3) The expression of LN-R and LN was associated with invasion and metastatisis, and can evaluate the prognoses of the NPSC. Part 5Objective:(1) To study the role of the micrangium and micro lymphangion in NPMT;(2) To provid the important parameter for the etiological factor,diagnosis, treatment and prognosis of the NPMT.Methods:The immunohistchemical technique was used to detected the expression of CD34 and D2-40 in cases of NPSC.Results:(1) The positive rate of the CD34 in NPMT was significantly higher than in para-cancer tissues and non-cancer tissues(P＜0.01),and the expression of D2-40 was no significance(P＞0.05);(2) There wasn't a significance on the expression of the CD34 and D2-40 in endepidermis and soft connective tissue of the NPMT(P＞0.05),and in different encroachment degree of the NPMT(P＞0.05)(3) The cluster analysis can be used in MVD counted number in NPMT.According to cluster analysis,the MVD counted number can be clustering two group:≥24 and＜24.The analytic result of the cluster is the consonance with MVD counted number.Conclusion:(1) The micrangium perhaps participate in regulation of occurrenc in NPMT,and the micro lymphangion may not be associated with tumorgenesis of NPMT;(2) The micrangium and micro lymphangion maybe play homoioplastic role in different pathotype of the NPMT;(3) The micrangium and micro lymphangion may not be associated with development of NPMT;(4) The cluster analysis can be used in exploratory study about the MVD counted number in NPMT.It can simplify MVD counted number and reduce relative accuracy.