Study On The Effect Of Honeysuckle Forsythia Powder And Extract Of It's Separately Groups Made By Nature-flavor Of Herbs On Apoptosis Induced By Influenza Virus Infection

Objective:Do the study on the separately groups of Honeysuckle Forsythia Powder on the basis of compatibility of medicine flavor-nature according to the theory of correlation between nature-flavor of herbs and their efficacy and to the doctors'thinking methods on clinical symptom-complex. Through observing MDCK cell which anti-IV infects of Honeysuckle Forsythia Powder and extract of it's separately groups. Explore the mechanism of how Honeysuckle Forsythia Powder can anti IV﹑main organism effect and excellent proportion of each herb in powder from the angles of anti IV,leading to produce Interferon and restrain apoptosis. Offer theory support and experimental basis for the anti IV effect of Honeysuckle Forsythia Powder from every target and segment.Methods:1. Prepare extract of Honeysuckle Forsythia Powder and it's separately groups by use of alcohol solution .2. Make infected mice model by use of inoculating IV in cavitasnasi, make the hemagglutination inhibition titer of virus in mice lung tissue observe target. Observe correlation between compatibility of every groups and the drug efficacy3. Make infecting vitro models in three ways which are infect first-take medicine then, take medicine first-infect then and take medicine when infect. Use CPE and MTT to observe the effect Honeysuckle Forsythia Powder and extract of it's separately groups made by nature-flavor of herbs take on apoptosis induced by infection of influenza virus4. Use DNA- agarose gel electrophoresis, TUNEL to examine apoptosis. Examine expression of apoptosis Fas/Fasl by use of Immunity group reduction. Observe the effect Honeysuckle Forsythia Powder and extract of it's separately groups made by nature-flavor of herbs take on apoptosis induced by infection of influenza virus5. Make infected mice model. Examine quantity of IV and content of IV by RT-PCR and ELISA, Observe the effect Honeysuckle Forsythia Powder and extract of it's separately groups made by nature-flavor of herbs take on apoptosis induced by infection of influenza virus with observing external features of mice, lung tissue pathological section, morphology and lung index.6. Examine expression of apoptosis Fas/Fasl by use of TUNEL and Immunity group reduction. Observe the effect Honeysuckle Forsythia Powder and extract of it's separately groups made by nature-flavor of herbs on apoptosis induced by infection of influenza virus. Result:1. To employ intuition analyze and orthogonal analyze, know the essential ingredients in Honeysuckle Forsythia Powder are Flos lonicerae ,forsythia suspense ,arctium lappa, bamboo leaf ,jingjiesui,gancao,jiegeng,mint(p<0.05),expect dandouchi(>0.05)2. Honeysuckle Forsythia Powder and it's separately groups can take treatmental measure on IV, It's effect is in direct proportion to concentration of medicine .The drug efficacy of Honeysuckle Forsythia Powder(1:1-1:4)and clear heat-toxic medicine(1:1-1:2 ) is similar to Ribavirin(p>0.05)Honeysuckle Forsythia Powder and it's separately groups can take prevention measure on IV .The drug efficacy of Honeysuckle Forsythia Powder(1:1-1:8), clear heat-toxic medicine(1:1-1:8 ) ,formulae that release the exterie with pungent -cool herbs (1:1-1:4 ) and formulae that release the exterie with pungent -warm herbs(1:1-1:8) is markly different to Ribavirin(p<0.05). Concentration of medicine is in direct proportion to ER. Honeysuckle Forsythia Powder and it's separately groups can directly kill IV. The drug efficacy of Honeysuckle Forsythia Powder, clear heat-toxic medicine is in direct proportion to concentration of medicine .The effect of Honeysuckle Forsythia Powder(1:1-1:2), clear heat-toxic medicine (1:1-1:4), formulae that release the exterie with pungent -cool herbs (1:1)which directly kill IV is better then Ribavirin (p<0.05),while formulae that release the exterie with pungent -warm herbs is similar to Ribavirin (p>0.05)3. Honeysuckle Forsythia Powder and it's separately groups and Ribavirin do not appear Apoptosis trapezoid atlas. It can reduce AI, apoptosis and light absorption of Fas (p<0.05). Compare to Ribavirin, the effect of Honeysuckle Forsythia Powder and clear heat-toxic medicine is similar to Ribavirin(p<0.05). But formulae that release the exterie with pungent–cool and warm herbs are quite different(p<0.05). Compare to IV, Honeysuckle Forsythia Powder, clear heat-toxic medicine, formulae that release the exterie with pungent -cool herbs and Ribavirin can be more induced the light absorption degree, but are different from each other (p<0.05) . Compare to Ribavirin, Honeysuckle Forsythia Powder and clear heat-toxic medicine are similar to it (p>0.05),but more different from formulae that release the exterie with pungent -cool herbs(p<0.05).4. Reconstructed model of mice IV pneumonia: the IV RNA examined in lung is expressed in bulk .Lung index increase. Pathological change is internior pneumonia:dropsy between pulmonary alveolei, smash by inflammatory cell .5. The expression of the total IV RNA examined in lung: Compare to IV,Honeysuckle Forsythia Powder and it's separately groups and Ribavirin are more different(p<0.05). Compare to Ribavirin, the effect of Honeysuckle Forsythia Powder is the same as clear heat-toxic medicine and formulae that release the exterie with pungent -cool herbs(p>0.05),is markedly different from formulae that release the exterie with pungent -warm herbs(p<0.05)6. Use RT-PCR to examine the expression of IFN-γ: Compare to virus infected groups, the effect of Honeysuckle Forsythia Powder is markedly different from clear heat-toxic magician , formulae that release the exterie with pungent -warm herbs and Ribavirin (p<0.05), is similar to formulae that release the exterie with pungent -warm herbs (p>0.05).Compare to Ribavirin.Honeysuckle Forsythia Powder, clear heat-toxic medicine and formulae that release the exterie with pungent -cool herbs are more effectual but formulae that release the exterie with pungent -cool herbs.Use ELISA to examine the expression of IFN-γ: it is increased in virus infected mice lung tissue (p<0.05). Compare to virus infected groups, The effect of Honeysuckle Forsythia Powder and clear heat-toxic medician, and Ribavirin (p<0.05) is more effectual, but formulae that release the exterie with pungent -warm herbs and with pungent -cool herbs (p>0.05): Compare to Ribavirin, Honeysuckle Forsythia Powder, clear heat-toxic medicine and formulae that release the exterie with pungent -cool herbs are more effectual but formulae that release the exterie with pungent -cool herbs. Compare to Ribavirin, Honeysuckle Forsythia Powder it's separately groups are more effectual (p<0.05)7. Infected can induce apoptosis in mice lung, more Fas/Fasl express(p<0.05). Honeysuckle Forsythia Powder and clear heat-toxic medicine can reduceAI, control apoptosis in lung(p<0.05), reduce expression of protein (p<0.05).Compare to Ribavirin Fasl, the effect of Honeysuckle Forsythia Powder is similar (p>0.05) but clear heat-toxic medicine(p<0.05). Compare to virus infected groups AI, formulae that release the exterie with pungent -warm herbs and with pungent -cool herbs are no different (p>0.05). Compare to Aciclovir Fas, formulae that release the exterie with pungent -warm herbs and with pungent -cool herbs are more different (p<0.05) and compare to normal groups, similar(p>0.05).Conclusion:1.From the angel of reducing the hemagglutination inhibition titer of the virus in lung , know the essential ingredient in Honeysuckle Forsythia Powder are Flos lonicerae ,forsythia suspense ,arctium lappa,jingjiesui,bamboo leaf,gancao,jiegeng ,dandouchi2. Honeysuckle Forsythia Powder and it's separately groups can take treatmenal and prevention measure on IV in MDCK cell and directly kill it. They can directly kill IV expect the less effectual formulae that release the exterie with pungent -warm herbs. In different ways of take medicine: the groups that first-take medicine then. Take medicine is more effectual on control virus3. MDCK cell Infected by IV can induce apoptosis .Honeysuckle Forsythia Powder can control it main through Fas/Fasl, and clear heat-toxic medicine make more act on it.4. Honeysuckle Forsythia Powder can directly kill IV, reduce damnification in lung and induce produce of Interferon. The former two are got from integration of clear heat-toxic medicine, formulae that release the exterie with pungent -warm herbs and with pungent -cool herbs ,the later one is main got from clear heat-toxic medicine5. Infected by IV can induce apoptosis in mice lung tissue .Honeysuckle Forsythia Powder can control it main through Fas/Fasl ,and clear heat-toxic medicine make more act on it.