The Study Of The Effect Of New Powder Of Lonicera And Forsythia And Preparation Of Arctium Fruit On Influenza Virus H1N1 Strain In Vivo And In Vitro

Title: The study of the effect of New Powder of Lonicera and Forsythia and preparation of arctium fruit on influenza virus H1N1 strain in vivo and in vitro.Speciality: Integrated basic TCM and Western Medicine.Author: Huang BisongTutor: Guo xingbo1 .Background and objective:Influenza is the first disease that is monitored in the whole world. It often breaks out in some areas. In our country, more than 80% people suffer from it twice to six times a year. Many countries pay a lot of attention and financing on the research of influenza virus because it frequently breaks out and causes thousands of people dying from it.One of the most important characters of influenza virus is constant changes in its antigen identity. In recently years, three types of influenza viruses are the main prevailing strains. They are influenza virus A (HlNl), influenza virus A (H3N2) and influenza virus B. This three types often emerge alternately for the change of virus' antigenicity and people's immune state.Powder of Lonicera and Forsythia is an old recipe which is usually used to prevent and treat influenza. It is recorded in treatise on differentiation and treatment of epidemic febrile diseases whose author is a famous doctor of Qing dynasty, Wu Jutong. It was used to treat the pyrexia diseases when the pathogen settles in the superficial layer or the upper Jiao originally. It is one of the representative recipes to release exterior syndrome with the drugs pungent in flavor and cool in property. The main drugs in it include honeysuckle flower, forsythia fruit, arctium fruit, lophatherum, schizonepeta spike, peppermint, licorice root, prepared soybean, platycodon root.Modern pharmacologic researches have ascertained that this recipe possesses outstanding efficacies of anti-virus, anti-bacteria, subducing inflammation, resisting allergy, inducing diaphoresis and strengthening immune ability. It is often modified to treat such diseases as influenza, acute tonsillitis, herpes angina, measles and epidemic parotitis, herpes simplex keratitis, viral myocarditis, pharyngoconjunctivitis and so on that caused by viruses.On the basis of the recipe, many preparations, including Pill of Lonicera and Forsythia for detoxification, Tablet of Lonicera and Forsythia for detoxification, Particle of Lonicera and Forsythia for detoxification and Mixture of Lonicera andForsythia for detoxification, have been produced and used in clinic. So it is one of those TCM recipes that are widely used and have definite efficacies.After reviewing the up-dated researches on the recipe, we found improves in powder of Lonicera and Forsythia were focused on two aspects: (1) Innovate in form. The forms of the recipe which is produced now include ointment, pill, powder, particle and syrup, etc. (2) Honeysuckle flower, peppermint and schizonepeta spike are prepared specifically according to their properties during producing.But we also noticed that the function and special property of arctium fruit didn't get deserving emphasis.The results of modern pharmacologic researches showed the main active ingredients in arctium fruit include arctiin and arctigenin which also are the main material base of many anti-influenza preparations. Both the materials are lignan compounds. They can inhibit the activity of sialidase of influenza viruses and prevent membrane fuse. And arctigenin have an excellent function on regulating immune reactions. So we believe the function of arctium fruit in powder of Lonicera and Forsythia is inneglectable.To extract the most arctiin and arctigenin, some special process must be taken during preparing. We studied the effect of new powder of Lonicera and Forsythia in which the arctium fruit were prepared specially and alcohol solution of arctium fruit and arctigenin on influenza virus by experiments in vivo and in vitro. We hope to prepared new preparations of powder of Lonicera and Forsythia of better efficacy or to find new monomer preparations that have definite efficacy and controllable quality for the treatment and prevention of influenza.Experiments and resultsInfect mice with H1N1 influenza virus and give drugs solutions with different concentration of new powder of Lonicera and Forsythia, powder of Lonicera and Forsythia, alcohol solution extraction of arctium fruit and arctigenin a day before infection. At the same experiment, control groups of model, normal and ribavirin were included. Observe the effect of those drugs on mice lung index.The results of experiments in vivo showed new powder of Lonicera and Forsythia can inhibit influenza virus and its efficacy is related to dose. The efficacy seem to have the trend of overcoming ribavirin and decoction of powder of Lonicera and Forsythia. Alcohol of arctium fruit and solution of arctigenin also have positive effect on influenza virus. And 0. 1ug/kg solution of arctigenin can't protect MDCK cells from the attack of influenza virus.Conclusion:...