A Study On Expression Of The PS2, PS2mRNA, P450arom, P450arommRNA In The Glandular Tissue Of The Pubertal Mammary Hypertrophy

Objective To investigate the expression status of the PS2, PS2mRNA, P450arom, P450arommRNA in breast tissue of pubertal mammary hypertrophy and then explore the possible etiology of pubertal mammary hypertrophy. Methods 15 patients were selected for pubertal mammary hypertrophy group. Breast hypertrophy tissue specimens were collected from the gland excised during reduction mammaplasty. The selection criterion was weight of the excised breast gland greater than 350 g. Patients were excluded if they had any of the following conditions: irregular menstrual cycle; history of pregnancy; hormonal treatment within at least 2 months; nipple discharge from either breast; cyst or fluid and milk concentration in the gland; family history of breast cancer; history of unilateral breast cancer, fibroadenoma or atypical hyperplasia. 10 patients with micromastia were used as a control group. The tissue specimens were obtained during the augmentation mammaplasty. 15 patients with pathologically simple fibroadenoma were used as another control group. The tissue specimens were obtained 3cm away from the fibroadenoma during the tumorectomy. These patients also met the conditions mentioned above. The reduction mammaplasty, augmentation mammaplasty and tumorectomy procedures were performed during the follicular phase of the menstrual cycle. Patient approval of participation in this study was obtained preoperatively. The expression of PS2 , PS2mRNA, P450arom, P450arommRNA was detected by Western Blot and RT-PCR in all the cases above. Results There was no significant difference among the pubertal mammary hypertrophy groups,micromastia groups and normal groups on the expression rates of PS2,PS2mRNA,P450arom,P450arommRNA. But among the positive cases, the expression of PS2,PS2mRNA,P450arom,P450arommRNA within breast tissue were respectively 0.515±0.112. 0.424±0.139. 0.198±0.060. 0.202±0.048 in pubertal mammary hypertrophy group; and 0.254±0.097,0.169±0.055,0.139±0.028,0.146±0.034 in micromastia group, and 0.214±0.078,0.163±0.051,0.144±0.072,0.159±0.068 in normal group. There was significant difference between the pubertal mammary hypertrophy and micromastia groups (P<0.01/ P<0. 01/P <0. 05/P < 0.05) and there was also significant difference between the pubertal mammary hypertrophy and normal groups (P<0.01/ P<0.01/P < 0.05/P <0.05) . Conclusion The expression of PS2, PS2mRNA, P450arom, P450arommRNA in pubertal mammary hypertrophy are significantly higher than in micromastia and in normal mammary glandular tissue. The pubertal mammary hypertrophy may be related to the expression status of PS2, PS2mRNA, P450arom, P450arommRNA within breast tissue.