Expression Of ER, CyclinD1 And Corresponding MRNA In Mammary Tissue Of Adolescent Mammary Hypertrophy And Observation Of Ultrastructure

The First Part: Expression of the ER, CyclinD1 in the Glandular Tissue of the Pubertal Mammary HypertrophyObjective To investigate the expression status of the ER, CyclinD1 in breast tissue of pubertal mammary hypertrophy and then explore the possible etiology of pubertal mammary hypertrophy. Methods 15 patients were selected for pubertal mammary hypertrophy group. Breast hypertrophy tissue specimens were collected from the gland excised during reduction mammaplasty. The selection criterion was weight of the excised breast gland greater than 350 g. Patients were excluded if they had any of the following conditions: irregular menstrual cycle; history of pregnancy; hormonal treatment within at least 2 months; nipple discharge from either breast; cyst or fluid and milk concentration in the gland; family history of breast cancer; history of unilateral breast cancer, fibroadenoma or pathologically atypical hyperplasia. 10 patients with micromastia were used as a control group A. 15 patients with normal breast tissue were used as a control group B. The tissue specimens were obtained during the augmentation mammaplasty. These patients also met the conditions mentioned above. The patients in three groups were strictly selected. Their menstrual cycles were normal. The reduction mammaplasty and augmentation mammaplasty procedures were performed during the follicular phase of the menstrual cycle. Patient approval of participation in this study was obtained preoperatively. The expression of ER, CyclinD1 was detected by western-blot in 15 cases of pubertal mammary hypertrophy, 15 normal breast tissue and 10 cases of micromastia. Results There was significant difference between the expression of ER, CyclinD1 within breast tissue in pubertal mammary hypertrophy and in micromastia. (P<0.01). There was significant difference between the expression of ER, CyclinD1 within breast tissue in pubertal mammary hypertrophy and in normal breast tissue. (P<0.01). There was no difference between the expression of ER, CyclinD1 within breast tissue in micromastia and in normal breast tissue. (P>0.05). Conclusion The expression of ER, CyclinD1 in pubertal mammary hypertrophy are higher significantly than in micromastia and normal breast tissue. The pubertal mammary hypertrophy may be related to the expression status of ER, CyclinD1 within breast tissue.The Second Part: Expression of the ER, CyclinD1 mRNA in the Glandular Tissue of the Pubertal Mammary HypertrophyObjective To investigate the expression status of the ER, CyclinD1 mRNA in breast tissue of pubertal mammary hypertrophy and then explore the possible etiology of pubertal mammary hypertrophy. Methods The patients were grouped just like the first part and the condition and age are the same. The expression of ER, CyclinD1 mRNA was detected by RT-PCR in 15 cases of pubertal mammary hypertrophy, 15 normal breast tissue and 10 cases of micromastia. Results There was significant difference between the expression of ER, CyclinD1 mRNA within breast tissue in pubertal mammary hypertrophy and in micromastia. (P<0.01). There was significant difference between the expression of ER, CyclinD1 mRNA within breast tissue in pubertal mammary hypertrophy and in normal breast tissue. (P< 0.01). There was no difference between the expression of ER, CyclinD1 mRNA within breast tissue in micromastia and in normal breast tissue. (P>0.05). Conclusion The expression of ER, CyclinD1 mRNA in pubertal mammary hypertrophy are higher significantly than in micromastia and normal breast tissue. It strengthened the assumption that the pubertal mammary hypertrophy may be related to the expression status of ER, CyclinD1 within breast tissue.The Third Part: Ultrastructure of the Glandular Tissue of the Pubertal Mammary Hypertrophy Objective To observe the ultrastructure in breast tissue of pubertal mammary hypertrophy and then explore the possible etiology of pubertal mammary hypertrophy. Methods The patients were grouped just like the first part and the condition and age are the same. The ultrastructure in breast tissue of pubertal mammary hypertrophy tissue, normal breast tissue and micromastia tissue were observed with transmission electron microscope. Results We can find fibroblast and collagen fibres in all three groups. There was more collagen fibres in pubertal mammary hypertrophy than in normal breast and micromastia tissue. The nucleolus of fibroblast in pubertal mammary hypertrophy is more big than in the normal breast and micromastia tissue. Conclusion The nucleolus of fibroblast in pubertal mammary hypertrophy is more big than in the mormal breast and micromastia tissue. There was more collagen fibres in pubertal mammary hypertrophy than in normal breast and micromastia tissue.