Studies On The Bioactive Differences And Essences In Different Preparations And Compatibilities Of Rhizoma Coptidis Based On Biothermodynamics

Theory of property of Traditional Chinese Medicine(TCM) is the foundation of research and application of TCM,and be also considered as focus and bottlenecks in TCM modernization.Property that is the core of TCM can explain bioactivity of TCD clearly.The traditional preparation and compatibility can change the property of TCD in different degree,while it change the bioactivity of TCD.In order to evaluate the effect of preparation and compatibility on bioactivity/property of TCD and discover its mechanism and possible material basis,based on thermodynamic theory, associated with pharmacological and chemical method,the bioactive difference and active essences in different processed products of Rhizoma Coptidis and complex prescription combined Rhizoma Coptidis with Fructus Evodiae were studied in this dissertation.The main results of research were as follows:1.The action of different processed products of Rhizoma Coptidis (Cuzhihuanglian,Danzhihuanglian,Yanzhihuanglian,Wuyuhuanglian, Jiangzhihuanglian and Jiuzhihuanglian) and the complex prescription combined Rhizoma Coptidis with Fructus Evodiae(Zuojinwan,Ganlusan,Zhuyuwan, Fanzuojinwan) on three bacteria(Escherichia coli,Staphylococcus aureus,Shigella dysenteriae) was investigated with biothermodynamics in this research.All the samples could restrain the bacteria growth and metabolism in different levels. According to thermokinetic equation,the rate constant k and the heat output were showed quantitatively.The results showed that all the samples could restrain the growth of bacteria and the biothermokinetic parameters were changed in different levels.Drags with cool property could make the organisms decreasing the rate constant k and decreasing heat output(Q) remarkably,while drugs with warm property could make the organisms increasing the rate constant k and increasing heat output(Q) a little.There was a stable difference between them.Additionally,the inhibitory effect of alkaloids of Rhizoma Coptidis on Shigella dysenteriae was quantitatively investigated by microcalorimetry.The experimental results indicated that the sequence of anti-microbial activities of the three alkaloids was:berberine＞coptisine＞palmatine.All the results above-mentioned were matched with the conclusions reported in literatures.2.Using routine pharmacological method,the effect of inhibiting bacteria and eliminating the heat syndrome of different processed products of Rhizoma Coptidis and the complex prescription combined Rhizoma Coptidis with Fructus Evodiae was studied.The results were consistent with the above results of biothermodynamics.So, the thermodynamic method could be used as an antibiotic active screening method in TCD study.3.By HPLC,five kinds of berberine type alkaloids:berberine,palmatine, coptisine,epiberberine and jatrorrhizine in different processed products of Coptidis Rhizoma can be separated completely,and the contents of alkaloids were determined in this paper.In the meantime,the contents of six main alkaloids including berberine, palmatine,coptisine,epiberberine,jatrorrhizine and rutaecarpine of different complex prescription were determined.What's more,HPLC fingerprints of different samples of Coptidis Rhizoma were established with different HPLC method.4.Using statistical method,the relation among threee experimental methods (Biothermodynamics,chemical analysis and routine pharmacology) was analyzed in this study.The correlation analysis of P-t curve of different processed products of Coptidis Rhizoma and HPLC plots of five alkaloids indicated the growth rate constant k of bacteria inhibited by preparation was interrelated with contents of berberine, palmatine and coptisine.While the correlation analysis of P-t curve of different complex prescription of Coptidis Rhizoma and HPLC plots of the main alkaloids indicated the growth rate constant k of bacteria inhibited was interrelated with the contents of berberine and jatrorrhizine.Both of which could be regarded as the main active essences.The results showed that biothermodynamics,whose characteristics are micromount,high effective,real-time and so on,could be an effective method to evaluate the bioactivity of TCD.In this dissertation,the differences of bioactivity of preparation and compatibility of Coptidis Rhizoma were explained.The method combined with TCM theory and biothermodynamic theory could provide new method for Four Property evaluation, treatment principle,quality evaluation,effective substances screening and research and development of new drug.