Angelica Fritillaria Flavescens Decoction Experimental Chronic Bacterial Prostatitis

There are three parts in this thesis:Literature Reviews,Theoretical Research and Experimental Research.1 Literature ReviewsThrough the comprehensive literature of the chronic bacterial prostatitis (CBP) at home and abroad in latest over ten years,the progress in clinical epidemiology,pathogeny,medicine and methods of treatment were grasped.The pharmacology of herbs in Dangguibeimukushen pill(DGBMKS pill) and the clinical application of DGBMKS pill also were summarized.Based on these researches,the foundation of this project was laid.The morbility of CBP is increasing worldwide and the disease age is getting younger,but the satisfactory effects can't be gained through antibiotics and CBP easily has a relapse.Chinese medicine can not only adjust the whole condition of the organism,but also cure the regional pathological changes of prostate,so it has the distinct advantages.DGBMKS pill is mainly applied to treat the infection of urinary and reproductive systoms and tbe therapeutic efficacy is well.But there aren't the literature of the experimental and pharmacological researches on DGBMKS pill,so these researches should be carried out.2 Theoretical ResearchBased on the guiding of Chinese medical theories and the literature reviews, the relationships between the dampness-heat,blood stasis and CBP were explored.The occurance of CBP is closely related to dampness-heat,blood stasis and blood deficiency.The Zheng of dampness-heat with blood stasis is the common Zheng style of CBP.The principle of treatment is clearing away dampness -heat,activating blood circulation to dissipate blood stasis,warming and tonifying.According to the literature on the herbs of DGBMKS pill,its characteristics were analyzed.These are the herbs pungent in flavour,warm in nature and having the functions of clearing and dispersing are comprised;there are the herbs of clearing away dampness in company with moistening dryness and treating blood in company with regulating Qi in this prescription;this prescription can adjust blood and Qi and give consideration to the upper and the lower of body;it is brief and refined.The part showed the project was scientific and feasible and gave the theoretical support to the project.3 Experimental Research Objective:(1)To observe and estimate the effect of Dangguibeimukushen decoction(DGBMKS decoction) on CBP;(2)To explore the mechanisms of DGBMKS decoction treating CBP through the experimental research.Methods:60 male,250±20g,Sprague-Dawley rats were randomly divided into 6 groups(10 in each):the control group(A),the model group(B),the western medicine group(C),the high(D),middle(E) and low(F)-dose groups of DGBMKS decoction.After 3 days of adaptively feeding,the CBP models were established in the latter 5 groups by injecting E.coli into the dorsal lobes of the prostate while the control group being injected 0.9%NS instead of E.coli.Two weeks Iater,DGBMKS decoction was perfused into the rat stomachs of D,E and F groups.The density of high-dose was 1.1667g/ml,the middle was 0.5833g/ml and the low was 0.2917g/ml.These were 14 times,7 times and 3.5 times of the adult dose which was DANGGUI 15g,ZHEBEIMU 20g and KUSHEN 15g in clinic respectively.Norfloxacin suspension was administrated to C group.Its density was 0.0093g/ml,whichwas 7 times of the adult dose—0.8g.While the same volume distilled water was administrated to the control group and the model group.The intragastric administration was 1ml/100g/d,once daily and for four weeks.Items detected:(1)the size,hardness,color and adhesion of the prostates; (2) the histopathological changes of the prostates;(3) White blood cell(WBC) count and the density of Small particle of lecithin(SPL) in the prostates;(4)the whole blood viscosity;(5)the contents of Superoxide dismutase(SOD) and malondialdehyde(MDA) in the prostates;(6)the expression of Tumor Necrosis Factor-α(TNF-α) and Intercellular adhesion molecule-1(ICAM-1) by immunohistochemistry; (7)the contents of Dihydrotestosterone(DHT),Interleukin-1β(IL-1β),Interleukin-2(IL-2),Interleukin-6(IL-6),Interleukin-8(IL-8),Secreted Immunoglobulin A(SIgA) in the prostates by radioimmunoassay (RIA);(8)the mRNA gene expression of IL-1β,TNF-αin the prostates through Reverse transcription polymerase chain reaction(RT-PCR).Results:(1)The extent of disease and adhesion of the prostates were significantly decreased;The inflammatory cell inflatration and the hyperplasia of fibrous tissue were significantly diminished and the secretion in prostate was significantly increased;the numbers of WBC was significantly decreased while the density of SPL was significantly increased by DGBMKS decoction.According to the data of experiments,the high-dose DGBMKS decoction had the best effect on CBP. (2)The whole blood viscosity was significantly decreased;the contents of SOD were significantly increased and MDA were significantly decreased;the content of DHT was significantly decreased;the content of SIgA was not statistically significant;the expression of ICAM-1 was significantly decreased;the content of IL-2 was significantly increased and that of IL-8 was significantly decreased;the content of IL-6 was decreased,but it was not statistically significant;the mRNA gene expression of TNF-αand IL-1βwere significantly inhibited by DGBMKS decoction.(3)The high-dose DGBMKS decoction was better effective in decreasing the whole blood viscosity,increasing the contents of SOD and IL-2,decreasing the immunohistochemistry expressiones of ICAM-1 and TNF-α,decreasing the contents of IL-1β,IL-8 and DHT and inhibiting mRNA gene expressiones of TNF-αand IL-1βthan the positive medicine(Norfloxacin).Conclusion:(1)Through the modern and scientific methods;the favourable effect of DGBMKS decoction on CBP was verified.This illustrated the certainty of DGBMKS decoction curing CBP.(2)The mechanisms of DGBMKS decoction on treating CBP were expounded on the levels of the whole,cell and gene.The mechanisms lay on:①reducing inflammation, depressing the hyperplasia of fibrous tissue and sterilizing;②recovering the secretion of prostate,improving microcirculation and permeation of medicine;③antioxidating and removing free radical;④decreasing DHT;⑤promoting immune function and adjusting the cytokine.The project provided the precise evidences to administrate DGBMKS decoction.The practical significance in clinical and the creativeness were demonstrated.But the mechanisms of DGBMKS decoction adjusting IL-6 and SIgA need to be studied further.