Studies On Chemical Constituents And Pharmacological Activities Of The Leaf Of Corylus Heterophylla Fisch

In this paper, the chemical constituents and pharmacological activities of the leaf of Corylus heterophylla Fisch were studied.According to the references, a few compounds were isolated and identified as so far, such as steroid, triterpenoid, and so on. Only few references were reported about the chemical constituents from the leaves of Corylus heterophylla Fisch. Modern pharmacyological studies showed that the herb had the effects of benefiting vital energy, promoting appetite and improving eyesight. In order to find out more new chemical constituents furtherly in the leaves of Corylus heterophylla Fisch and develop the accurate pharmacological activites, comprehensive research were carried out in this paper. The research methods and results were showed as follow:The dried leaves of Corylus heterophylla Fisch, collected in Mt.Changbai, extracted with 85% alcohol. In order to be concentrated, the alcohol solution were evaporated with distillation method at normal pressure.Then the concentrated solution was dissolved with water. Next the water solution was extracted with petroleum ether, chloroform, acetic ether and n-butyl alcohol respectively at room temperature. Finally, thirty two compounds were obtained from chloroform part, acetic ether part and n-butyl alcohol part respectively, which were dealt with silica gel chromatogramphy by using several kinds of solvent systems, HPLC and recrystallizing repeatly. Among of them, thirty compounds' structures were identified on the basis of chemical and spectroscopic evidence (1HNMR, 13CNMR, 2DNMR, HRMS), they are 7,8,10-trimethoxy-4,5-dihydrophenan thro[2,3]dixole ( 1 ), 1,3-dihydroxy-5,8-dimethoxy- 2,4-bis(3-methylbut-2-enyl)-xanthen-9-on(e2),gartanin,(3),kaempferol,(4),2,7-dihydroxy- 3,5-dimethoxy-9, 10-dihydrophe nanthrene, 6-methoxycoelonin,(5),2,3,5,7- tetramethoxy-9, 10-dihydrophenanthrene,(6),kaempferol-3-O-β-D-glucopyranoside,(7),kaempferol-3- qpiofur-anosylglucopyrano side, apioliquiritin,(8),8-deoxygartanin,(9),8-hydroxycudra- xanthone G,(10),7-hydroxy dihydroflavonone-4'-O-β-D-glucopyranoside,(11),7,4'- dehydroxyflavonone,(12),7-hydroxydihydroflavonone-4'-O-β-D-glucopy ranoside, apioli- quiritin,(13),2,4-dihydroxy chalcone-4'-O-β-D-glucopyranoside,(14),2,4-dihydro- xychalcone-4'-O-β-D-glucopyrano side,fructofuranoside ,( 15 ), daucosterol ,( 16 ), 4,4'-dimethoxy-5,5'-bibenzolignin[d] [2,3]dixole,(17),2',6'-diethylbiphenyl-2-ol,(18),β-Sitosterol,(19),2,4,4'- trihydroxychalcone,(20),7,22-dien-3- ergosterol,( 21), ethyl glucopyran oside,( 22),α-D-(6-O-sinapoyl)-glucopy ranosyl-β-D- (3-O-sinapoyl)-fructofuranose,(23),4-hydroxy-3-methoxy benzoic acid, vanillic acid,(24),ρ-hydroxybenzoic acid,(25),pyrocatechol,(26),palmitic acid,(27),2-methyl acrylaldehyde ,( 28 ), phenylacetic acid ,( 29 ), 6,7-dimethoxy-5-hydroxycoumarin ,(30).Among of them, compound 3-18 and 24-30 are all separated from this plant firstly. Both compound 1 and 2 are new chemical compounds.The effects of ethyl acetate extract of hazel leaves (EEHL) and gartanin on blood lipid metabolism were investigated in experimental hyperlipidemia mice. The results showed that EEHL could decrease the level of TG level and gartanin could decrease the level of TC level in blood serum of experimental hyperlipidemia mice.The new compound 2 had been carried out anti-metalloproteinase activity with the method of fluorescence enzyme labeling for the first time. The experimental results showed that compound 2 had significant inhibitory effect on Matrix Metalloproteinase (MMP). Because the metalloproteinase inhibitor has the effect of anti-cancer, the results could provide a scientific basis for developing compound 2 as anti-cancer drugs.