Cooling Blood Drug Experiment On The Effects Of Retinal Neovascularization

Purpos:to study the therapeutic liangxuehuoxue effect on the supression of retinal neovascularizationMethods:the ECV304cells were cultured with different concentrations of liangxuehuoxue,The proliferation rate of endothelial cells are assayed with MTT methods,the level of VEGF was tested.The animal model of oxygen-induced retinopathy was made by subjecting postnal mice to hyperoxic conditions taken retinal angiogram with ink.Quantified the degree of hyperoxia-induced neocascularization in serial paraffin cross sections by counting the number of vascular cell nuclei on vitreal side of the intemal membrane,the expression of HIF-1 and NF-κB in the oxygen-induced retina with immuhistochemistry were observed,also the weight,the weight of brain and length were measureded.Results:Liangxuehuoxue could inhibit the proliferation of ECV304 cells and the expression of VEGF.Afier the mouse were induced by oxygen,the endothelial cell nuclei increased and HIF-1,NF-κB went up obviously,The length,weight,and weight of brain got down.Liangxuehuoxue caused the endothelial cell nuclei reduced and HIF-1,NF-κB descended.Coneulsions:liangxuehuoxue inhibits the ECV304 cells proliferation and oxygen-induced intravitreal retinal angiogenesis by down-regulated HIF-1and NF-κB related to the angiogenesis.Therefore,the treatment of liangxuehuoxue will provide certain experiment basis for the clinical practice.