The Relationship Between The Therapy Of Radioactive Cerebral Edema With Methylprednisolone And Dexamethasone And The Expression Of CD18 MRNA

Partâ… Establishment of Rat Cell Line C6 Brain Glioma Model with StereotaxisObjectiveTo establish an brain glioma models of C6 cells in Wistar rats with different methods and to study and compare the growth patterns of the tumors formed in the wistar rat brain.MethodsRat C6 glioma cells were cultured in vitro. 10μl of cell suspension containing 1.0×10⁵ C6 cells/10μl or 1.0×10⁶ C6 cells/10μl or 1.0×10⁷ C6 cells/10μl was stereotaxically inoculated into the right caudate nucleus of the rat brain. The moving and survival satates of rats with gliomas were observed. The examinations of the tumors formation, volumes, metastassis and histopathology were performend. The expression of glial fibrillary acidic protein and vascular endothelial growth factor in the tumors and the microvessel density were detected by immunohistochemistry.ResultsAll the tested rats of different inoculate dose developed brain tumors without extracranial metastasis. The rat C6 gliomas resembled histopathclogical features of human glioma. The tumors contained many cells with remarkable cytoplasmic and nuclear polymorphism, and pathological mitosis figures. The scattered positive expression of vascular endothelial growth factor was observed in tumor cells. There was the strongly positive expression of glial fibrillary acidic protein in the tumors.ConclusionsThe successful rate of C6 brain tumor model established by the intracerebral inoculation of C6 cells is very high in rats. The tumor established in the experiment is similar to human glioma in histopathological features and it can be used as a good model to study the glioma. The tumors model established by inoculation of different dose of C6 cells can be selected according to different needs of different experiments because their growth speeds are different from each other. Partâ…¡The Relationship Between the Radioactive Cerebral Edema of the Rat of C6 Brain Glioma Model and the Expression of CD18 mRNAObjectiveTo study the extent of radioactive cerebral edema and the expression of CD18 of the rat neutrophilic granulocyte.Methods40 male Wistar rats were divided into 2 groups at random (n=20). Group A was irradiated with Co60, group B was normal control. Planted glioma cells into brains of rats of group A and B, and treated groups A with Co60 after 15 days. The water content of the brains of the rats and the expression of CD18 of the rats neutrophils were measured 7d after irradiation. Collect the normal brain tissue and the tumor tissue about 1mm3 and observed blood capillary on electron microscope.ResultsThe water content of the rats brain: There were significant differences between group A and B (P<0.05).The level of CD18 mRNA: There were significant differences between group A and B (P<0.05). The blood capillary of tumor tissue was much than the normal brain tissue, the blood capillary of tumor tissue of irradiated rat was injuried and have edemata around it.ConclusionRadioactive ray can lead to the brain edema and the development of radioactive cerebral edema is related by change of the expression of CD18 in rats. Partâ…¢The Relationship Between the Therapy of Radioactive Cerebral Edema with Methylprednisolone and Dexamethasone and the Expression of CD18 mRNAObjectiveTo study the effect of methylprednisolone and dexamethasone to radioactive cerebral edema and the expression of CD18 of the rat neutrophilic granulocyte. MethodsOne hundred male Wistar rats were divided into 5 groups atrandom (n=20). Group A was irradiated with 30 mg/kg methylprednisolone, group B was irradiated with 10 mg/kg methylprednisolone, group C was irradiated with 5 mg/kg dexamethasone, group D was irradiated without glucocorticoid, and group E was normal control. Planted glioma cells into brains of rats of group A,B,C,D and E, and treated groups A,B,C,D with Co60 after 15 days. All groups gave glucocorticoid or normal saline beginning 3d before and ending 6d after irradiation(iv, qd), The water content of the brains of the rats and the expression of CD18 of the rats neutrophils were measured 7d after irradiation.ResultsThe water content of the rats brain: There were significant differences between group D,E and group A,B,C(P<0.05) , group D and group E(P<0.05), group C and group A,B(P<0.05); There was no significant difference between group A and B(P>0.05). The level of CD18 mRNA: There were significant differences between group D,E and groups A,B,C(P<0.05); group D and group E(P<0.05), group C and group A,B(P<0.05); There was no significant difference between group A and B(P>0.05).ConclusionMethylprednisolone and dexamethasone can both protect the radiation-induced injury and brain edema changes by inhibiting the expression of CD18 in rats. and the methylprednisolone is more efficient than dexamethasone. Partâ…£The Development of Radioactive Cerebral Edema and the Curative Effect of MethylprednisoloneObjectiveTo study the method of radioactive cerebral edema and the effectiveness of methylprednisolone and dexamethasone.Methods63 patients of radioactive cerebral edema were reviewed, the percentage of various kinds entity, the time from radiotherapied to find radioactive cerebral edema were statisticsed. The change of clinical symptom and the ambit of cerebral edema treated with methylprednisolone(group A) or dexamethasone(group B) were compared.ResultsIn 63 patients, have 17 meningeoma(26.98%), 12 arteriovenous malformation(19.05%), 12 cavernous angioma(19.05%), 10 glioma(15.87%), 6 tumor in cerebral ventricle(9.52%), 2 hypophyseoma(3.17%), 2 acoustic nerve tumor(3.17%), 1 tumor of apophysis cerebri(1.59%), 1 metastatic tumor(1.59%). 0mon-36mon from radiotherapied, the symptoms of radioactive cerebral edema appeared. Average is 8.1 month. The effective power of group A is 92.30%, The effective power of group B is 88.89%, showing no significant difference. But other than in group B, significant differences of ambit of cerebral edema between pretherapy and post-treatment were detected in group A(P<0.05).ConclusionsParasagittal sinus meningioms, arteriovenous malformation, cavernous angioma, glioma and tumor in cerebral ventricle have more chance of radioactive cerebral edema. Radioactive cerebral edema often betide 1 year after radiotherapiy, especially in 1st month and 5^th mon-7^th mon. Methylprednisolone and dexamethasone can both protect the radiation-induced injury. And the methylprednisolone is more efficient than dexamethasone in imageology.