The Preliminary Investigation Of The Effect Of Chinese Traditional Medicine-ErHuangSan On Cervicitis And Cervical Intraepithelial Neoplasia

PartⅠThe clinical investigation on cervical disease of WuhanObjective To investigate the distribution of cervical disease of Wuhan to provide the clinical information for the research of the therapy and prevention of cervical disease.Methods A total of 2634 patients in our hospital had been examined by TCT and TBS from December 2004 toMay 2005.The The abnormal cases which had been selected by TCT were detected by colposcopical pathological examination and Hybrid CaptureⅡHPV testing.Results 493 ( 18.71% ) abnormal smears were found out of 2635 cases, in which atypical squamous cell of undetermined significance (ASCUS) 271cases (10.28% ),low grade squamous intraep ithelial lesion (LSIL) 94 cases ( 3.57% ),high grade squamous intraep ithelial lesion (HSIL) 14 cases (0.53% ) , squamous cell carcinoma 1 case (0.04% ) , adenocarcinoma endometrium 1 case (0104% ).CINⅠ,CINⅡ,CINⅢcases which were diagnosed by colposcopical pathological examination were 31.18% (53 /170 ),23.53% (40 /170 ),7.06% (12 /170 ).HPV infection which was detected by TCT were 105 cases (3.98% ).High risk HPV-positive cases which were detected by Hybrid CaptureⅡHPV testing were 74 cases.Conclusion The disorder of cervical is the normal disease of women.The CIN and HPV infection are the dominant factors which damage the healthy of women. PartⅡThe clinical observation of the effect of Chinese traditional medicine -ErHuangSan on high risk HPV infection CINⅠ,Ⅱand and the expression of IFN-γ,TNF-α, IL-4,IL-10Objective To observe the clinical effect of ErHuangSan on high risk HPV infection CINⅠ,Ⅱand the expession of IFN-γ,TNF-α, IL-4,IL-10.Methods 158 cases of high risk HPV infection patients with CINⅠ,Ⅱwere employed in the study for 3 months. 94 cases were treated by Er Huang San .30 cases were treated byα-2b interferon pessus as the positive control .34 cases were treated by nothing as the blank control.All the cases were detected by Hybrid Cap tureⅡHPV testing, colposcopical pathological examination before and after the treatment.The ELISA was employed to detect the expression of IFN-γ,TNF-αIL-4,IL-10 in Erhuangsan group before and after the administering of ErHuangSan.Results The therapeutic efficacy of ErHuangSan andα-2b interferon pessus on high risk HPV infection were 75.6% and 73.3% respectively. The therapeutic efficacy on CINⅠ,Ⅱwere 72.4% and 73.3%。The The therapeutic efficacy of ErHuangSan andα-2b interferon pessus had no significant difference and better than the blank group obviously.After the treatment of ErHuangSan,the expression of IFN-γand TNF-αraised but the IL-4 and IL-10 deduced.Conclusion ErHuangSan has the therapeutic efftect on the cervical intraepithelial neopasia. ErHuangSan can raise the expression of Th1 cytokine,so that the cellular immunity predominate to eliminate the virus. PartⅢThe effect of Chinese traditional medicine-ErHuangSan on the morphopathology and the expression of TNF-α,IL-10 in cervical tissues of cervicitis ratsObjective To investigate the effective method to build an animal model of cervicitis for the fundamental and clinical research of cervicitis. To investigate the effect of ErHuangSan on the morphopathology and the expression of TNF-α,IL-10 in cervical tissues of cervicitis rats before and after the treatment of ErHuangSan.Preliminary investigate the effect of ErHuangSan on local immunologic function of cervical tissues of Wistar rats.Methods The vagina of experimental Wistar rats were injected the 20% mucilage of phenol to build the cervicitis model,the cervicitis rats were divided randomly to three groups: negative control group, ErHuangSan group and XiaoMiShuan(positive control) group which were treated by blank mucilage ,ErHuangSan and XiaoMiShuan respectively.The rats never be treated were employed as the blank control.the manifestation and the vaginal secretion of rats were observed.The cervical slices were HE stained, the morphological change and the inflammation level were observed by microscope.3.The immunohistochemistry(S-P) and reverse transcription-polymerase chain raction(RT-PCR) were employed to detect the expression of TNF-α,IL-10 in cervical tissues.Results The thickness vaginal secretion could be seen at the collar extension of vagina of the negative control group.The animals of negative control group massed, pilo-erection, Compared with the blank control group,the dgree of cervical inflammation and the quanity inflammatory cells of negative control group had the significant difference(P<0.05). Compared with the negative control group, morphopathology of cervical and the quanity of inflammatory cells of the ErHuangSan group and XiaoMiShuan group had the significant difference (P<0.05). there was no diffrence between the blank control group, ErHuangSan group and XiaoMiShuan group. The immunohistochemistry(s-p) and reverse transcription-polymerase chain raction(RT-PCR) were employed to detect the expression of TNF-α.The absorbency in blank control group,negative group and ErHuangSan group were 0.1436±0.0027,0.1769±0.0035,0.1443±0.0012.Compared with blank control group ,the expression of TNF-αin negative control group increased obviously(P<0.01). Compared with negative control group,the expression of TNF-αin ErHuangSan group reduced(P<0.01).The absorbency value of IL-10 in blank control group,negative control group and ErHuangSan group were 0.1336±0.0031,0.1386±0.0049,0.1547±0.0051.The expression of IL-10 in ErHuangSan group increased.there was significant difference between the ErHuangSan group and negative control group(P<0.01). Reverse transcription-polymerase chain raction(RT-PCR) was employed to detect the expression of TNF-αand IL-10.The expression of TNF-αmRNA in blank control group,negative control group and ErHuangSan medicine group were 0.103±0.031,0.979±0.045,0.719±0.026 respectivily Compared with blank control group ,the expression of TNF-αin negative control group increased obviously(P<0.01). Compared with negative control group,the expression of TNF-αin ErHuangSan group reduced(P<0.01).The expreesion of IL-10 were0.172±0.019,0.487±0.026,0.726±0.021,in blank control group,negative control group and ErHuangSan group. The expression of IL-10 in ErHuangSan group increased.there was significant difference between the ErHuangSan group and negative control group(P<0.01).Conclusion: The injection of 20% phenol mucilage to the vagina of experiment rats can result in the inflammation of cervical . The ErHuangSan was conducive to the healing of the cervical. Along with the occurrence of cevititis ,the expression of cytokine TNF-αand IL-10 raised..The traditional Chinese traditional medicine-ErHuangSan can reduce the expression of TNF-αand raise the expression of IL-10 to adjust the immunity balance of cervical which is good for the convalescence of cervicitis.