Cattle Wood Anticancer Active Ingredient Research

854 Chinese herbs targeting at RXR had been screened in vitro.The extract of Cratoxylum cochinchinense(Lour.)Blume was found effectively.This thesis focused on the isolation and structure elucidation of anticancer constituents from the Chinese herb and the pure compounds were carried on with further pharmacological studies.Cratoxylum cochinchinense(Lour.)Blume,called"Huangniumu"in the producing area of China,is a shrub belonging to the family Guttiferae,26 xanthones,and 2 other kind of compounds were isolated from the stem of C.cochinchinense by bioassay-guided fractionation.Their structures were determined on the basis of physical chemical properties and spectroscopic analysis.Compounds 1-14 were new ones,while 19,21,23 and 24 were isolated from the genus of Cratoxylum for the first time.According to the results of the pharmacological screen,several xanthones were found to be able to induct the expression of NUR77 and inhibit the growth of various cancer cell lines and the transactivity of RXR.As NUR77 was a potent apoptotic member of the steroid/thyroid hormone receptor superfamily,the results suggested that induction of NUR77 and RXR might be a mechanism by which xanthones exert their antitumor effects,and more investigations needed to be conducted to ascertain.