Differentiation And Function Of Adipose-derived Mesenchymal Stem Cells In Rat Diabetic Retinopathy

PURPOSETo investigate the effects of AMSCs on BRB in diabetic rats and the distribution in the organs.To identify the AMSCs' potential of differentiation in the retina.METHODSDiabetes was induced by streptozotocin in a single intraperitoneal injection.Human AMSCs were isolated,cultured and then injected intravenously.Close monitor on the blood glucose during the following four weeks.BRB function was evaluated by Evans Blue infusion.The distribution and differentiation of AMSCs was observed by immunofluorescence.RESULTSSignificant decrease in the blood glucose and improvement of BRB function by measuring Evans Blue leakage was observed after AMSCs intervention.The existence of AMSCs was found in the retina,with expression of photoreceptor and astrocyte-specific markers(Rhodopsin and GFAP,respectively).AMSCs were also present in other tissues,such as pancreas,liver,kidney,spleen and cornea.CONCLUSIONSAMSCs have the potential to differentiate into photoreceptors and astrocytes in diabetic retinopathy,and repair the BRB defect.The decreased level of blood glucose may relate to the local distribution of AMSCs in the pancreas.