The Effects And Its Mechanism Of Activating Yang On Rats With Hepatic Sinusoid Capillarization By Ameliorating The Status Of Phlegm And Blood Stasis Handing Over

ObjectiveTo confer the mechanism and treatment principles of coexistence of phlegm and blood stasis from the angle of the lose function of yang qi,we want to establish a pathology model for phlegm and blood staiss handing over,and to study the effects and its mechanism of activating yang ameliorating the status of phlegm and blood stasis handing over by observing the changes on livers of model rats and the changes of depositing collagen,Mmp-2,Timp-2,liver function and so on.Methods1.50 SD male rats,choose randomly 7 rats as normal group,the remainder were fed the mixture of alcohol and fat for 111 days.Then we gave a hypodermic injection of 40%CCL₄ 0.3ml/100g in the legs of the remainder for 4 weeks,and observed the livers of model rats by electron microscopes to evaluat the model of hepatic sinusoid capillarizatin in rats.2.At the same time of injecting,34 rats were randomly divided into model group(9 rats),fistular onion stalk extraction group (9 rats),Chinese herbal medicine contrast group(7 rats),western medicine group(9 rats).And fed physiological saline,fistular onion stalk extraction,colchicine,Danshen injection immediately for 19 weeks.SABC was used to assay the levels of C-IIIIIIIIV in the livers of rats.Western-blot was used to assay the levels of Mmp-2,Timp-2 in the livers of rats.Radioimmunoassay was used to assay the levels of LN,HA,PIIIP,CIV in the blood of rats.Hydrolysis was used to assay the levels of HyP in the livers of rats and so on.Results1.The method of SABC shows:CIV was weakly expressed in the livers of normal group.It was strongly expressed in the livers of model group.The method of Western-blot shows:the levers of CIV were expressed higher in model group and treatment groups than in normal group.The levers of CIVwere expressed lower in treatment groups than in model group.The levers of CIV in fistular onion stalk extraction group were lower expressed than in other treatment groups.2.The method of hydrolysis shows:fistular onion stalk extraction could reduce the levers of HyP in model group.The levers of HyP in western medicine group were not different from the levers in model group.3.The method of radioimmunoassay shows:the levers of LN,HA,PIIIP,CIV in the blood of model group were higher than the levers in the blood of normal group.The levers in treatment groups were lower than the levers in model group.4.The method of western-blot and RT-PCR shows:the levers of protein and gene of Mmp-2 in livers of model group increased obviously.The levers of protein and gene of Timp-2 in livers of model group were increased obviously too.Fistular onion stalk extraction could reduce the levers.5.The method of biochemistry shows:the levers of ALT,AST in the blood of model group were higher than the levers in the blood of normal group.The levers of ALB,A/G in the blood of model group were lower than the levers in the blood of normal group.Fistular onion stalk extraction and Chinese herbal medicine could finerly ameliorate the liver function of model rats than western medicine.6.The method of biochemistry shows:the levers of TC,TG,HDL-C,LDL-C in the blood of model group were lower than the levers in the blood of normal group.Fistular onion stalk extraction and Chinese herbal medicine could raise the levers.And Fistular onion stalk extraction could obviously raise the levers of HDL-C.Western medicine had not this function.Conclusion1.With regard to the reasons and treatment principles of coexistence of phlegm and blood stasis,we bring up 2 points of view:â‘ Yang qi's deprived of its function of dredging and making fluent is a key to the occurrence and development of phlegm and blood staiss handing over.â‘¡Activating yang has 2 meanings:one is dredging the blocked phlegm and blood,the other is to interrupt vicious circle of the state of an illness and turn bad process to virtuous circle by making yang qi fluent.2.Established successfully a pathology model for phlegm and blood staiss handing over for the first time.And surround those views,verified fistular onion stalk extraction can ameliorates phlegm and blood staiss handing over on rats with hepatic sinusoid capillarizatin.Further experiments proved that the function of dredging of fistular onion stalk extraction maybe have something to do with it could reduce the expressiveness of CIVand the levers of HyP,LN,HA,PIIIP,CIV.The function of making fluent of fistular onion stalk extraction maybe have something to do with it could reduce the expressiveness of Mmp-2x Timp-2,encourage a new balance of Mmp-2/Timp-2,ameliorate the function of model rats.3.The experiment supplied a visual and objective provement for activating yang ameliorateing phlegm and blood staiss handing over.It enlarges a train of thought for treat coexistence of phlegm and blood stasis.