| In this study, the innovative opinion was that "gout disease in the liver, spleen and kidney dysfunction in liver-based, liver toxicity by muddy, Yun steaming hot and humid are its key pathogenic factor". Liu wei tongfeng-Drink was selected clinical effective drugs to clear liver discharge muddiness, Tonifying splenorenal, both temporary and permanent cures. In order to further assess its efficacy, we set up in rats with acute gouty arthritis and hyperuricemia quail model, experimental study.Objective: To explore Liu wei tongfeng-Drink gouty arthritis treatment and prevention of its recurrence mechanism.Methods: (1) Acute gouty arthritis experimental research: Applications Coderre Ways classic model, made of acute gouty arthritis rat models, respectively Liu wei tongfeng-Drink, colchicine, aceclofenac for gavage treatment, the rats tested joints gait, as well as the circumference of its joint swelling and histopathological changes. Using enzyme-linked immunosorbent assay (ELISA) detection methods, MMP-3, IFN-γ, IL-4 expression changes was observed in rat articular of every group; At the same time, the analgesic effect of the treatment group were observed through the mouse writhing test. (2) Hyperuricemia Experimental studies: application to adenine give quail gavage method, building hyperuricemia quail model, respectively, to Liu wei tongfeng-Drink, allopurinol, benzbromarone for gavage treatment, observation quail serum uric acid and xanthine oxidase Change the content.Results: (1) Liu wei tongfeng-Drink, colchicine and aceclofenac significantly improve the gait of rats (P<0.05). (2) Liu wei tongfeng-Drink, colchicine, aceclofenac can inhibit acute gouty arthritis joint swelling in rats, joint circumference in the treatment group compared with the model group that has differences (P<0.05), the no difference between the treatment groups (P>0.05). (3) Pathological observation showed that Liu wei tongfeng-Drink, colchicine and aceclofenac can improve the model of gouty arthritis patients the pathological changes of articular synovitis. (4) MMP-3 levels of the joints surrounding tissue in the blank group, the treatment groups were lower than the model group, the difference between meaningful (P<0.05); all is no difference between the treatment groups (P>0.05). (5) IFN-γlevels of the joints surrounding tissue in the blank group, the treatment groups were lower than the model group, the difference between meaningful (P<0.05), between the treatment groups no significant difference (P>0.05). (6) IL-4 content of joints surrounding tissue in the blank group, the treatment groups were higher than model group, the difference meaningless (P>0.05).(7) The treatment groups are able to reduce the acetic acid-induced mouse abdominal pain, compared with the model group, were significantly different (P<0.01), between the treatment groups compared with no difference (P>0.05). (8) Quail model of hyperuricemia model have been successfully set up, model of serum uric acid was significantly higher than the blank group, the difference was significant (P<0.01). (9) Serum uric acid of Liu wei tongfeng-Drink group, Allopurinol and benzbromarone group than it's group before treatment were significantly decreased, the difference was statistically significant (respectively P<0.05, P <0.01, P<0.01 ); drug treatment, Liu wei tongfeng-Drink group, Allopurinol group and benzbromarone group than those in model group, serum uric acid decreased, the difference was significant (respectively P<0.05, P<0.01, P<0.01); while between the treatment groups compared with no significant differences (all P>0.05). (10) Blood XOD at Liu wei tongfeng-Drink group, Allopurinol group than in their pre-medication has declined markedly, the difference was statistically significant (respectively P<0.05, P<0.01); XOD at Benzbromarone group was lower than its medication before, the fall there was no significant difference (P>0.05); After their drug treatment, blood XOD at Liu wei tongfeng-Drink group, Allopurinol group is lower than those in model group, the difference was statistically significant (P<0.05, P<0.01); blood XOD in benzbromarone group compared with the model group, that was no statistical significance (P>0.05); blood XOD at Liu wei tongfeng-Drink gout than allopurinol group, which is lower than benzbromarone group , the differences were statistically significant (P<0.05); Allopurinol group was significantly lower than blood XOD benzbromarone group, there is significant difference (P<0.01).Conclusion: (1) Liu wei tongfeng-Drink for gouty arthritis rat model has a good anti-inflammatory and analgesic effect of swelling. (2) Model of gouty arthritis have MMP-3, IFN-γexpression in it's joint surrounding tissue, no obvious IL-4 expression. (3)Liu wei tongfeng-Drink can reduce surrounding tissue inflammatory response by inhibiting MMP-3, IFN-γactivity of gouty arthritis model articulation surrounding tissue, which may be related to IL-4 expression has nothing to do. (4) The success of the quail hyperuricemia model was set up, their blood uric acid and xanthine oxidase increased. (5) Liu wei tongfeng-Drink can reduce serum uric acid and serum xanthine oxidase activity of hyperuricemia quail model. (6) Liu wei tongfeng-Drink can be effective prevention and treatment of gouty arthritis.
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