| ObjectiveAlong with the clinical use of azole drug in treating candidal vagin itis,the azole drug bears the developing resistance,the mechanism of fl uconazole to Candida albicans lies in the medicine enteing in the cell an d correlates with gene or some of key enzyme to play the roles of killing or suppressing the strain,lts drug resistance production also is connect ed the relative medicine gene expression or the variation result;It is d ifficult for patients to recover,already brought to the widespread atten tion;How to reduce the patient to azole drug resistance,or develops the treatment t to candidal vaginitis the new method,or the new medicine,r educes the patient pain,already was the major issue which the general me dicine worker faced.The research discovered that,the effect of clinical Chinese medicine treatment regarding candidal vaginitis is good,may redu ce the patient pain obviously;According to beforehand research,the effe ct of Xianglian solution treating the candidal vaginitis is remarkable,a nd has the obvious inhibitory action to the Candida albicans.The research about the resistance to azole drug of Candida albicans a lready arrived the molecular level under the modern molecular biology tec hnology,and in the unceasing,the scientists overseas already located to special genes now,and carry on the multi-positions research to special gene;Domestic research at present basically emphatically in whole functi on gene research,and elaborating the whole change of genes to the mechan ism of resistance.This goal of the research mainly appeals tothe modern molecular biolo gy technology,reveals the molecular mechanism of Xianglian solution in t he gene level to Candida albicans,including Candida albicans sensitive a nd resistance strain,analysis different strain of the function gene expr ession under the outside cleaning solution,carries on multi-target super elevation flux screening using the gene analysis correlation technology t o the Chinese native medicine wash-out side,constructs Xianglian solutio n gene function mechanism;The effect of Chinese medicine treating to be good,but its concrete action mechanism is obscure,has the similar actio n mechanism equally with fluconazole? has own specificity revelation targ et spot?Regarding repeatedly candidal vaginitis and the strain Candida albica ns the external use of Xianglian solution has good effect,what is it in the molecular action level mechanism also? It is worth our well research, provides the evidence for the Chinese medicine preparation standardizati on;It is significant for the research of Chinese medicine and moves towa rds the world.Method1 Fluconazole induces the SC5314 standard strain to resistance strain for the short-term,and observe the gene difference expression.Compare the resistance strain of short-term and the original generation of strain, understands the drug resistance in the gene stratification plane formati on.2 Xianglian solution was used to the SC5314 short-term resistance str ain,and observe the gene difference expression.Compare the two results, one is the difference of short-term SC5314 resistance strain versus the strain was used by Xianglian solution,the other result is the difference SC5314 standard strain to resistance strain for the short-term,by the c ontrast,we can know the detail spots on the function of the Xianglian so lution and can analysis the genes deeply.3 Compare the result about the laboratory induction strain used by Xia nglian solution and clinical resistance strain used by Xianglian solution. And analysis the result,to find the common difference genes,and discov er the function special target and carries on the multianalysis.4 Compare the uniform of NCCLS M27-A3,the S3 MIC observation method and the glucose consumption trial survey MIC method research.Uses the glu cose consumption trial to survey the MIC of Candida albicans processed by Xianglian solution.Result1 We may know the result of MIC of fluconazole to three kind of strai n,Candida albicans strain SC5314 MIC is 0.4883mg/ml processed by Xiangli an solution.The fluconazole function in SC5314 MIC is 0.25μg/ml;We may in fer in vitro experiment of suppressing Candida albicans SC5314,Xianglian solution 0.4883mg/ml and fluconazole 0.25μg/ml are equal.2 Along with Xianglian solution density rise,the experimental two ki nd of strain various glucose density is higher,explained the glucose con sumption are less,after taking the logarithm of the medicine density and carring on the analysis,we discovered the logarithm of the medicine den sity and each glucose density presents the linear relations;Compare the two kind of strain linear relations,and found the uniformity to two kind of strain functions.Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.ConclusionXianglian solution,takes one kind of hospital self-restraint externa 1 use medicament,although its development utilization has about 10 years, but also only is limits to the hospital use,the Xianglian solution not yet starts regarding Candida albicans MIC research,therefore is also far the standard criteria MIC to Candida albicans.Therefore,this experimen t has also carried on the preliminary exploration to Candida albicans of the Xianglian solution MIC determination,provokes the thought for later research.In this experiment,Xianglian solution in experimental strain S C5314 its MIC is 0.4883mg/ml.The fluconazole function in SC5314 MIC is 0. 25μg/ml,We can basically infer in vitro experiment Candida albicans SC53 14,Xianglian solution 0.4883mg/ml and fluconazole 0.25μg/ml have the equ al potency.Xianglian solution and fluconazole to the Candida albicans glucose co nsumption influence and the analysis research discovered Xianglian soluti on has the uniformity to two kind of strain functions.Along with the flu conazole solution density rise,the experimental two kind of strain vario us glucose density is higher,the explanation glucose consumption are les s;Carries on the analysis comparison to under the fluconazole function t wo kind of strain linear relations,discovered that the two strains have the nonuniformity.This possible to explain two medicines the function mec hanisms are dissimilar,fluconazole main function in cytochrome P450 ster ol synzyme way,but Xianglian solution function is unknown actually in an y way;Perhaps this is Xianglian solution and the fluconazole function un similarity,will wait for further studies in later.Observation of the fluconazole resistance strain SC5314 and sensitive strain carring on the gene chip research discovery,its difference gene expression mainly concentrates in:The code proteinase body and the prote in hydroltyic enzyme gene,in the code sugar fat metabolism process is co nnected the protein gene,the cell cycle correlation gene,the duplicatio n and the translation adjustment correlation gene,the stress response co rrelation gene,the line plastochondria correlation gene,the cell wall f unction related gene;Obviously with formerly studied reports about the r eaiatance genes the expression was difference,through this experiment di scovered fluconazole induced SC5314 to bear the medicine strain from sens itive in the process,the gene expression difference was the duplication and the translation,metabolism,the proteinase body gene;The cell drug resistance formation is many genes affects together,and possible to affe ct,possibly is not several sole genes which the way different causes has caused the drug resistance formation,will wait for in the later further research.Campare the result of the sensitive to resistance strain and the stra in was processed by Xianglian solution,changes gene is codes the stress r esponse family protein the gene:HSP30,HSP60,TIF51.3;RPT3,RPT5,HCT5. 3eoc,UBP6.3 belongs to a code proteinase body gene race,and CDC1,CGR1, CCN1 belongs to the code cell cycle related protein gene,the PPR1,SWI1, MEF2,TUF1,TIF3 gene code involves the duplication and the translation adjustment related protein;As a whole explained Xianglian solution funct ion gene mainly with the cell stress correlation gene,the recognition be ars the medicine gene to come under the influence to be small.The observation of the different resistance strains processed by Xian glian solution,we discovery the gene which affects mainly is codes hot s hock protein gene SSB1,MGE1,the serine/threonine protein activating enz yme gene,codes KNS1,RPS13.3,RPS22,PUP2,YST1.exon2,RPL10E and RPL28. 3f in the proteinase body family gene,the code is responsible to regulat e copies and translates gene PRT1,IPF776,GCD11,CAP1,TIF35,CAM1.exon2, EFT2,EFT3.The research of Xianglian solution affecting fluconazole inducing str ain and clinical reaistance strain indicated that after Xianglian solutio n processing,Candida albicans come from stress to steady state,when the Candida albicans is in bears the medicine condition,the heat is in shoc k the protein expression ascension,Xianglian solution function secondary fever shock protein expression reduces:Xianglian solution function can restore the cell normal function after the strain,the serine/threonine p rotein activating enzyme revivification is normal.Simultaneously improve s the cell load,when the cell is in bears the medicine condition,the ce ll internal proteinase body family function strengthens,degrades the cor relation protein,the promotion cell foreign environment compatibility,a fter Xianglian solution function,in the cell the environment is stable, the stress responded the related protein reduces,the proteinase body cor relation gene expression level reduces.Certainly most essential is Xiang lian solution can improve bears the medicine correlation gene the express ion,restores the cell the sensitivity.This research main discovery strain bears the medicine is the complex process,is not several sole gene function result,in this research,the recognition bears the medicine gene not to discover the expression diffe rence,involved the gene as well as Candida albicans which the cell drug resistance formed the cleaning solution function target in the recent yea rs literature has not discovered the similar report;Perhaps the Chinese medicine has its own unique function target spot;In brief,Xianglian sol ution appears its unique superiority in this experiment,no matter is sup presses the Candida albicans the growth aspect,a gene target aspect,and the former literature result is different,is worth later detailed resea rch.
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