Antifungal Resistance Analysis Of Xianglian Lotion Against 40 Vaginal Isolates Strains Of Candida Albicans

Objectives:To priliminarily research into the range of both the minimum inhibitory concentration (MIC) and the minimum fungi cidal concentration (MFC) of Xianglian lotion against 40 strains of Candida Albicans in vaginal isolates. And analyze the medical history data of 40 patients with Vulvovaginal Candidiasis (called VVC for short). Thereby to totally and objectively comprehend and probe into various aspects of general clinical situations as well as diagnosis and therapy on the VVC disease.Methods:In the experimental respect, the MICs of both fluconazole and Xinaglian lotion as well as its MFCs against 40 strains of C. albicans in vaginal isolates were all measured by broth microdilution method according to document《Re-ference Method for Broth Dilution Susceptibility Testing of Yeast》(M27-A Document) published by the American National Institution for Clinical Lab-oratory Standards(CLSI) in 1997. The Candida strain of quality control(QC) is Candida krusei ATCC6258. In the clinical respect, collect the clinical history of 40 patients with VVC who conformed to the subsumption standard of etiology. Then analyze the results by the collection. Afterwards know about the general clinical situations of VVC disease and probe into the aspect on its development of diagnosis and therapy, in order to roughly understand va-rious VVC information on the clinical characters and guidelines of diagnosis and treatment.Result:1. The experiment result is that the Candida strain of QC, Candida krusei ATCC6258, is under the control of the MICs range according to the document M27-A. The MICs range of 40 Candida strains are:Fluconazole 4.00±2.94μg/ml, Xinaglian lotion 3.20±2.83mg/ml. The MFCs range of Xianglian lotion is 31.25～62.5mg/ml. Besides, there's no prominent difference between the bacteriostatic effects of these two med-ications (P>0.05).2. On the clinical aspect, the results of patients'history collection are: The ages of 40 patients with VVC disease, all of whom have the life history, are 32.4±10.15 (20～55 years old); 20% people in total for subsequent visits. The main symptoms of patients are pruritus of vulva and vagina (28/40,70 %), erubescent on the vulva and vagina (30/40,75%), cervical lightly erosion (14/40,35%). Second are leucorrhea increase (22/40,55%), bean curd residue alike of the stiff excretions in vagina (20/40,50%). Medicine choices si-tuation is that Xianglian lotion 52.5%(21/40), imidazole class medicines 82.5%(33/40), mycetin cataplasma 27.5%(11/40), the purely medicine law ac-ception of the vagina 45%(18/40), external use integrated oral treatment 55% (22/40), Chinese integrated western medicine 87.5%(35/40).Conclusion:The susceptibility tests of antifungal agents in vitro proved that Xiang-lian lotion in some concentration range has its own approving inhibitory and fungicidal effects against C. albicans and, in comparision with fluco-nazole and Xianglian lotion, there's few difference of the bacterio-static effects between them two. The WC is, after the bacterial vaginosis, the second common disease of genital tract infection, which affects a majority of women's phy-sical and psychology health a lot and mainly occurs among women of child-bearing age. Sexual behavior is one of the important ways of the Cross infection on the WC disease. C. albicans is still the main pathogenic fungus. The results of patients'history collection shows that imidazole class medicines (as Miconazole Nitrate Cream),polyene medicines (as mycetin cataplasma) and Xianglian lotion are in common use to treat WC in our hospital. The medicine law acception of the vagina,external use integrated oral treatment and the way of Chinese integrated western medicine are the first choices of therapies for WC patients in the outpatients of the gynecology and the dermatology & venereology departments of our hospital.