| Objective:1.To study the result of FFA,choroidal tissue and expression of FⅧR:Ag in choroidal neovascularization(CNV),to investigate the progress and characters of CNV induced by krypton laser in BN rat.2.To study the expression and the relationship of vascular endothelial growth factor(VEGF) and pigment epithelium-derived factor(PEDF) in choroidal neovascularization.3.To study the inhibition of experimental CNV by Decoction of Liangxue Huayu4.To study the clinical therapeutic effect of age-related macular degeneration(AMD) by Decoction of Liangxue Huayu.Methods:1.Four groups of 24 BN rats were photocoagulated with 20 spots by krypton laser in single eye.1 weeks,2 weeks,3 weeks,4 weeks after photocoagulation,photo of eye ground and FFA examination were performed,the quantities of CNV was measured by choroidal tissue analysis in 2 BN rats every week.The other 4 rats were executed with eyes excising,paraffin sections were made by these samples and observing the pathology and expression of FⅧR:Ag.2.Study the growth of CNV in four weeks and the expression of VEGF and PEDF in it.3.Three groups of 48 BN rats were photocoagulated by krypton laser in both eyes. One day after photocoagulation,one group of 16 rats received intragastric administration of Decoction of Liangxue Huayu,and the other group intravitreal injection of 2μl triamcinolone as control group.1 weeks to 4 weeks after photocoagulation,FFA was performed first.1 rat was vaso-infused with fluorescein isothiocyanate dextran(FITC-D) and the area of CNV was estimated.Other samples were made to paraffin sections and estimated the expression of FⅧR:Ag,VEGF and PEDE4.Study the therapeutic effect of 109 eyes in 90 AMD patients by Decoction of Liangxue Huayu in 6 months.Analysis the results of eye sight,area of exudation and hemorrhage,area of total affected,foveal thickness and total macular volume and evaluate the untoward reaction.Results:1.FFA,measurement of CNV area by stretched preparation of choroid and pathology, expression of FⅧR:Ag showed the increasing area of experimental CNV in 4 weeks (P＜0.05).2.The expression of VEGF increasing and PEDF decreasing in the formation of experimental CNV(P＜0.05).3.FFA and stretched preparation of choroid for quantitative analysis of CNV showed fewer area of CNV than blank and control groups at 4 weeks(P＜0.05).While the expression of FⅧR:Ag and VEGF were fewer than blank and control groups at 4 weeks(P＜0.05),the expression of PEDF was higher than those groups from 1 week to 4 weeks(P＜0.05).4.After 3 months treating AMD patients by Decoction of Liangxue Huayu,eye sight increased in 25 eyes(22.94%),stabled in 74 eyes(67.89%) and decreased in 10 eyes(9.17%); after 6 months,eye sight increased in 32 eyes(29.36%),stabled in 60 eyes(55.05%) and decreased in 17 eyes(15.6%).And there is no statistical meaning with the area of hemorrhage and exudation,area of total affected,foveal thickness and total macular volume.Conclusion:1.Krypton laser photocoagulation inducing CNV of BN rat may use for studying on AMD because its similarity in the growth,pathology with human's CNV.2.FFA examination,immunohustochemistry of FⅧR:Ag and stretched preparation of choroid for half-quantitative analysis of CNV were reliable index estimating CNV.3.Mechanism of CNV formation involves the balance of the expression of VEGF and PEDF.4.Decoction of Liangxue Huayu can inhibit the growth of experimental CNV through decreasing the expression of VEGF and increasing the expression of PEDF.5.Decoction of Liangxue Huayu can increased or stable most eye sight of exudation AMD patients,stabilization edema of macula and the area of hemorrhage and exudation, area of total affected with few untoward reaction.It's a safe and effective therapy.
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