Experiment Study On Endothelin And Angiotensin Systems Expression In Prostate Tissue And Prostate Stroma Cells Influenced By LBX Capsule

Benign prostatic hyperplasia(BPH) is a kind of histopathology disease, pathologic feature is prostate stroma and epithelia hyperplasia.BPH is a common and frequently disease occurring illness in older men.It is reported that BPH occurs in more than 50%of men over 60years,15%-30%of patients causing lower urinary tract symptoms(LUTS).Clinical symptom of BPH involved two aspects:first is stimulant symptom, (frequent micturition,urgency,nocturia,urge incontinence);second is block symptom, (hesitancy,intermittency,dribbling,acute uroschesis).In pathophysiology,BPH is performed as bladder outlet obstruction(BOO).The pathogeny of BOO involved two fields:first is static factor,prostate stroma and epithelia hyperplasia extrude posterior urethra induce mechanical obstruction;second is dynamic factor,α₁-adrenoreceptor distributing in bladder neck,capsula prostatica,prostate smooth muscle be activated, smooth muscle contraction,muscular tension enhancement,increased pressure of bladder neck and urethra,micturition resistence accretion induce dynamic obstruction.Clinical observations have been proved that nourishing qi and activating blood Chinese traditional medicine LBX capsule can reduce the volume of prostate,inhibit excessive hyperplasia of prostate,develop the quality of life and improve the dysuria symptoms.At the same time,the experimental study suggests that LBX can blockα₁-adrenoreceptor of cerebral cortex in canines,inhibit the 5-α-reductase activity of prostate,increase the NOS content of prostate,reduce the bFGF expression,restrain endothelial cell division of prostate and DNA synthesis and promote the apoptosis of prostatic epithelial cell,rebuild prostate hyperplasia cell dynamic balance,restrain prostate stroma and smooth muscle cell proliferation or reduce fibroblastic translate into smooth muscle cell.LBX could improve the symptom of BOO obviously.Recently,more and more studies indicate that endothelin and angiotensin systems have important effection in pathogenesy of BPH.In this study,we use immunohistochemistry,enzyme linked immunosorbent assay and quantitative RT-PCR methods to detect endothelin-1(ET-1),endothelin receptor A(ETAR), angiotensinll(AngII),angio-tensin receptor-1(ATI) and angiotensin-converting enzyme(ACE) in rat prostate and in vitro human prostate stroma cell.The conclusion as follows:1.ET-1 and Angll have been detected in both normal rat prostate and hyperplasia rat prostate,mostly localized in glandular epithelium and a little stroma cells,in rat hyperplasia prostate its expression raised obviously.2.Content of ET-1 in hyperplasia rat prostate rised up obviously,LBX could reduce content of ET-1 in rat prostate,clue to LBX could restrain secretion of ET-1 in rat prostate.3.ETAR gene expression rised up in hyperplasia rat prostate,LBX could reduce expression of ETAR obviously,clue to LBX could bring down ET-1 and ETAR combination ratio and reduce their biological effect.4.LBX drug serum could reduce ETAR gene expression of in vitro human prostate stroma cell,clue to LBX could restrain generation of ETAR in human prostate stroma cell.5.Content of Angll in hyperplasia rat prostate rised up obviously,LBX could reduce content of Angll in rat prostate,clue to LBX could restrain secretion of Angll in rat prostate.6.AT1/ACE gene expression rised up in hyperplasia rat prostate,LBX could reduce expression of ATI/ACE obviously,clue to LBX could reduce Angll and AT1 combination ratio and reduce biological effect,at the same time,LBX could bring down expression of ACE to reduce Angll generation.7.LBX drug serum could reduce AT1/ACE gene expression of in vitro human prostate stroma cell,clue to LBX could restrain generation of AT1/ACE in human prostate stroma cell.