Investigation On Syndrome And Study Of Immunoregulation Of T Cell Subset And Red Cell Immunology By Ways Of Promoting Blood Flow And Dissipating Phlegm On Chronic Pelvic Inflammatory Disease

Objective:To explore the law of syndrome in chronic pelvic inflammatory disease (CPID) by studying of literatures and classical works and investigation on large sample.To explore the influence on immunologic function of T lymphocyte subpopulation and erythrocyte by ways of promoting blood flow and dissipating phlegm working on the model rats.Methods:1.To elaborate on pathogenesis and treatment according to syndrome differentiation of CPID based on literatures and classical works.2.To progress clinical investigation on syndrome in CPID by questionnaire and to analyze it by the statistical methods.3.The models of rats with CPID(Wistar female rats) were successfully made by infecting three various bacteria into the rats.96 female Wistar rats were randomly classified into the following different guoups:the normal group,the simulative model group,the model group,the low dose of Guizhifuling capsule group,the medium dose of Guizhifuling capsule group,the high dose of Guizhifuling capsule group and the Shaofuzhuyu capsule group,the Jingangteng group both as the control groups.The light microscope was used in the morphologic research.The method of Flow Cytometry(FCM) was used to detect the percentage of the amount of CD3~+T,CD4~+T,CD8~+T and CD4~+CD25~+ regulation T cell(CD~4+CD25~+Treg) in the spleens of the model rats with CPID,accordingly CD4~+T/CD8~+T ratio obtained by calculation.The method of Rosette formation Test was used to detect the RBC-C3bRR and RBC-ICR in the blood serum. Results:1.The main pathogenesis of CPID is stasis of blood and the main treatment is the ways of promoting blood flow and dissipating phlegm.2.CPID can be classified into five patterns of syndrome.The five patterns of syndrome are stagnation of blood with dampness and heat type,qi-stagnancy and blood stasis type,stagnancy of stagnation of blood type due to deficiency of kidney,stagnation of blood with dampness and cold type and deficiency of vital energy and blood stasis type with the percentages of 31.3,23.8,19.7,14.6 and 10.6.3.The low dose of Guizhifuling capsule group,the medium dose of Guizhifuling capsule group,the high dose of Guizhifuling capsule group and Shaofuzhuyu capsule group,which all obey the ways of promoting blood flow and dissipating phlegm and Jingangteng group which obeys the ways of clearing away the heat-evil and expelling superficial evils have curative effect upon on the model rats.The inflammatory extent of the uterine constitution has been alleviated.All treating medicines have significantly increased percentages amount of CD3~+,CD4~+T and CD4~+T/CD8~+T ratio in the spleens of the model rats and reduced percentages amount of CD8~+T in the spleens of the model rats. The medium and high dose of Guizhifuling capsules have significantly increased percentages amount of CD4~+CD25~+Treg in the spleens of the model rats.A negative correlation is found between CD4~+CD25~+Treg and CD4~+T.That is the same between CD4~+CD25~+Treg and CD4~+T/CD8~+T.Guizhifuling capsule and Shaofuzhuyu capsule have significantly increased RBC-C3bRR in the serum of the model rats and decreased RBC-ICR in the serum of the model rats.Conclusion:According to the investigation of syndrome and research on pathogenesis theory of CPID,we perform experiment by ways of promoting blood flow and dissipating phlegm working on the model rats.The abnormal and disorder immunologic function of T lymphocyte subpopulation and red blood cell plays an important part in the disease.The medicines whose chief therapeutic method is promoting blood flow and dissipating phlegm can alleviate inflammation,adjust immunity of T lymphocyte subpopulation and red blood cell.