The Effect Of Wubeizi Ointment On Clinic Keloid Therapy And Fibroblast Proliferation, Apoptosis And Procollagen Gene Expression In Vitro

Objective:To evaluate the clinic effect of Wubeizi Ointment(WBZ Ointment) on keloid and the effects of WBZ Ointment solution on fibroblasts proliferation,apoptosis,and typeⅠandⅢprocollagen gene expression.Method:39 patients with 82 keloids were selected,which were divided into 3 groups,treated respectively with WBZ Ointment,Heibu Ointment (HB Ointment) external use and Disprospan injection in in.jury skin,and the cumulative scoring were made before treatment and post treatment in 1^st month,3^rd month,and 6^th month respectively.6^th month late,the clinic effect were evaluated.In experiment research,keloid tissue of activity stage were selected for keloid-derived fibroblasts culture in vitro,and the WBZ Ointment solution were divided into three doses consisting of large, middle and small dose,which were applied to fibroblasts respectively. And the effects of the drugs on keloid fibroblasts proliferation was examined by MTT colorimetric cell proliferation assay,cell DNA period and apoptosis detected by flow cytometry,as well typeⅠandⅢprocollagen gene expression of keloid fibroblasts by mRNA-DNA dot blot hybridization.Results:1 The clinic effective rate of WBZ Ointment was 96.56%,which was equal to Disprospan treatment,both of them were better than HB Ointment(P＜0.05).And compared to Disprospan,WBZ Ointment had little side effects(P＜0.01) 2 WBZ Ointment solution restricted keloid fibroblasts proliferation with dose-dependent manner.3 WBZ Ointment solutions accelerated the keloid fibroblasts apoptosis.4 After WBZ Ointment solution application,the keloid fibroblasts which were in G2M stage and S stage increased,with S stage impaled,it suggested WBZ Ointment could restrict keloid fibroblasts proliferation.5 WBZ solution restricted typeⅠandⅢprocollagen gene expression of keloid fibroblasts significantly.Conclusion:WBZ Ointment has satisfied effect on keloid,and WBZ Ointment improved the lagged preparation and the defects of traditional ointment,such as easy to dry and fall off,short period to storage,and WBZ Ointment hasn't side effects like Disprospan.Therefore,it has some advantage on clinic application for keloid.The mechanism of WBZ Ointment for keloid involve in restricting keloid fibroblasts proliferation and typeⅠandⅢprocollagen gene expression,as well as accelerating keloid fibroblasts apoptosis.