Experimental Study Of Marsdenia Tenacissima Injection Combination With Platinum In Treatment Of Primary Liver Cancer

Primary liver cancer is one of the most common malignant tumor in China, a serious threat to human health and life. Because of hidden hit of the disease onset, progress rapidly, the low rate of early diagnosis, most patients had reached advanced stage of disease at the time of diagnosis. Surgical treatment is often lost opportunity with these patients and those can only palliative treatment of the symptomatic treatment and support. Consider the effect of chemotherapy was bad. easy to produce multi-drug resistance, have low efficiency, coupled with drug side effects of chemotherapy drugs, the slow progress of the study, much-needed breakthrough. Research on new drugs and efficient treatment of low toxicity and reasonable treatment in traditional Chinese and western medicine in order to improve the treatment of liver tumor are need to explore and overcome problems.Xiaoaiping Injection came from the rattan plant root, stem or leaf and was made by the modern process of Marsdenia tenacissima. It being used in clinical treatment of various types of cancer and cough, asthma and other diseases. But it was very weak in basic research and clinical research rather shallow, there have a requirement with depth and systematic study for Xiaoaiping injection and its mechanism of anti-liver cancer.The article reviewed the progress in the research of antitumor activities and mechanisms of Marsdenia tenacissima. Its origin producing area and antitumor constituents were analyzed. It provides theoretical evidences for study on constituents in this traditional Chinese medicine. Futher studies on the species in Marsdenia tenacissima are needed for better medicinal utilization. Experimental results showed that: Xiaoaiping injection inhibited liver cancer cells in vitro and in vivo, its inhibition increased with the time and concentration gradually. Xiaoaiping had anticancer effects of diversification and target many of the characteristics of cancer cells. It could increase cells in G₀/G₁ phase, decrease cells in G₂/M phase and morphological appeared on some of the performance of induction of apoptosis; It could inhibit the expression of Ki-67 protein, the inhibitor of apoptosis protein Bcl-2 expression decreased, Bax protein expression enhanced.Cisplatin was one of the essential drag in chemotherapy of hepatocellular carcinoma. It was restricted in clinical application because of its toxicity. To explore the different effect of different platinum complexes in human hepatocellular carcinoma cell line (HEPG2, SMMC-7721 and Bel-7402) by experiment in vitro, this experiment intent to dedicate evidence for choosing platinum complexes in clinical chemotherapy of hepatocellular carcinoma. To observe the cellular morphology after being given different platinum complexes. To calculate inhibitory concentration (IC50) and get cell growth curve by MTT. The cell cycle was analyzed by flow cytometry. We observed the decrease of cell growth, increase of particle of endochylema; increase of the depressant effect to human hepatocellular carcinoma cell line with the increase of time being given platinum complexes. Cisplatin and Oxaliplatin were better than lobaplatin, nedaplatin and carboplatin in the depressant effect to human hepatocellular carcinoma cell line. FCM analysis showed that there appeared similar change in cell cycle after being given platinum complexes. Those showed platinum complexes had depressant effect to human hepatocellular carcinoma cell line in vitro. Cisplatin was the essential drug and Oxaliplatin was another better choice for clinical chemotherapy of hepatocellular carcinoma. The two drags were prefered in chemotherapy of hepatocellular carcinoma.The role of the United experimental studies had shown that Xiaoaiping with platinum-based drags on liver cancer cells in vitro displayed synergy. Xiaoaiping needed to reach a certain dose level with the synergy of performance significantly. It could enhance platinum-based drags on inhibition of liver cancer. The mechanism of their synergy had yet to be studied in future.