Long-shaped Nutmeg Medicinal Research

Purpose:Nutmeg that carried in"Chinese Pharmacopoeia" 2005 versions as a Chinese herbal medicine with warming middle,lower qi functions was generally called the round nutmeg.It is used for the bellyache,dysentery,vomit symptoms.Myristicin and safrol which had certain toxicity in the nutmeg were higher.So the search of another Chinese herbal medicine with similar functions seemed to be particularly important.We wanted to Proceed the research under the Chinese medicine theories by the Modern science techniques.For the first time,we proceed the in-depth research of Myristica argentea(Myristica argentea Warb.),established optimal processing method and quantity standard,compared with chemistry compositions of round nutmeg,further investigated the volatile oil from processed products and pharmacological functions that influenced the cancer cell multiplication outside the body.The subject Provided the dependable basis for the developing research and clinical application of the Myristica argentea,gived a useful attempt for Chinese herbal medicine modernization.Material and method:1.medicinal materials:The round nutmeg(Mystica fragrans Houtt.) bought in Shenyang Medicine Material Company,identified by prof ZhaiYanJun from Liaoning University of Chinese Medicine.It was Dry mature kernel of the nutmeg from the nutmeg section.The Myristica argentea(Mystica argente Warb.) bought in Bozhou City Anhui Province,identified by prof ZhaiYanJun from Liaoning University of Chinese Medicine.It was Dry mature kernel of the Myristica argentea from the nutmeg section. 2.method:Confirmed the external appearance through general observations.Analysed the compositions of volatile oil from two kinds of nutmegs by TLC and HPLC methods. Separated the compositions of volatile oil from Myristica argentea and alcohol extract with the modern layer-analysis technique and method.Analysed the compositions of volatile oil from Myristica argentea by GC/MS methods.Determined Contents of various chemical elements by ICP-MS.Confirmed optimal processing method and technic of Myristica argentea by analyzing different processed products of Myristica argentea.Established quantity standard of Myristica argentea and its processed product according to "Chinese Pharmacopoeia" 2005 versions.Examine preparation evaluation and pharmacokinetic study of simvastatin self-emulsifying capsules.Compared The anti- tumor functions in tumor cells directly with two kinds of volatile oil.Results:1.The Myristica argentea was very different from the round nutmeg in length.2.The contents and compositions of two kinds of nutmegs were different.Myristica argentea had less volatile oil,less active compositions(methyleugenol,methylisoeugenol),more toxic composition(safrol),minimum amount of myristicin.3.The results showed that the optimal processing condition was that:Nutmeg was soaked with water for 6hr,then bran at 160-170℃for 25min.every 100kg nutmeg with 40kg wheat.4.This subject established quantity standard of Myristica argentea and the product roasted with wheat,and gived drafting explanation.5.According to SEDDS system,optimal prescription of self-emulsifying capsule was that: Volatile oil:Ethyl Oleate:Tween 80:Transcutol P(5:45:50:5)(w).And the dissolving curves matched up to the "Hixson-Crowell" formula.Self-emulsifying capsules were steady with good appearance,appropriate emulsifying speed,appropriate particle diameter,favorable bioavailability.6.The volatile oil from two kinds of Nutmegs had the inhibiting function to the multiplication of cancer cells(HepG-2,SGC-7901,KB) outside the body.And the inhibiting intensity depended on the dosage.Conclusion:Myristica argentea Can be used as medicinal materials of nutmeg herb with the research of Chemistry composition,processing method,quantity standard,pharmacological function, preparation studies.