The Preliminary Study Of Lactosaminated Nanopaticle Gene Carrier On Hepatoma Treatment

Objective:To prepare the galactosyated human serum albumin (gala-HSA) nanoparticles,which bind galactosyl and show good magnetic responsiveness,to carry chemotherapeutic drugs;and examine the properties of the gala-HSA nanoparticles by determing the number of galactose residues.Method:Galactosyated human serum albumin was prepared by covalent coupling lactose to albumin using reductive amination method. The Fe₃O₄magnetic nanoparticles prepared by the titration hydrolyzation, gala-HAS,magnetite and purified adriamycin hydrochloride were mixed by appropriate proportion.Thereafter,the nanoparticles preparation process included sonication,albumin heat polymerization,washing with ether.The number of galactose residues was determined by phenol-sulfuric colorimetric method.The content of nanoparticles was by measured by liquid chromatography.The gala-HAS nanoparticles were resolved into normal saline.The motions of the particles were observed in saline using light microscopy,and the intrinsic detailed structure was observed by transmission electron microscopy.Results:The average number of galactose residues was 32.the diameter the Fe₃O₄ magnetic nanoparticles ranged from 50nm to 100nm; And the average diameter of nanoparticles was 70 nm.The adriamycin content of galactosyated human serum albumin magnetic nanoparticles was 58.45ug/g;97.53%of the drug is trapped into nanoparticles;Under light microscopy,we could find that nanoparticles moved towards magnet rapidly in saline and gather together edge of to the side of magnet.If the magnet was moved away a certain distance,the speed of nanoparticles slowed down and aggregated and clusterd into longitudinally aligned short cylindrical chains.Under transmission electron microscopy,it was found the Fe₃O₄ was well-distribution in the galactosyated human serum albumin magnetic nanoparticles containing adriamycin.Conclusion:galactosyated human serum albumin magnetic nanopartics containing Adriamycin have high the number of galactose resideues and excellent stable target effect,realease of chemotherapeutic drug from the nanopartics can be controlled.It provided a reliable carrying drug system for the targeting treatment of cancer.