The Relationship Between CLOCK Gene Polymorphism And Major Depression Or Insomnia, And Sleep Ectroencephalogram Physiological Characteristics In Ptients With Major Depression And Insomnia

Objectives:1) To explore initially the relationship of CLOCK gene polymorphism and major depressive disorder and insomnia.The purpose is to suppose presumption as following:whether CLOCK may be the genetic vulnerable factor in major depression,major depression with sleep disorder and insomnia.2) To evaluate sleep clinical characters of major depression and insomnia by Pittsburgh Sleep Quality Index scale,and to detect multiplying channel sleep electroencephalogram,to explore sleep character between male and female patients, to compare the objective characters with subject characters in major depression and insomnia.3) To evaluate Hamilton's depression scale(HAMD) and Hamilton anxiety scale(HAMA) in depression and insomnia,and investigate the relationship between HAMD and PSG,between HAMA and PSG,between PSQI and PSG in depression and insomnia.Methods:1) To adopt case control study,155 MDD(concluding 133 MDD with sleep disorder) and 105 insomnia were assessed with general health state of oneself—developing questionnaire,HAMA,HAMD,150 healthy controls were assessed with HAMD;25 MDD patients and 15 insomnia and 14healthy controls were assessed with PSQI.2) To detect the CLOCK gene Tag-SNP polymorphism in 155 MDD(concluding 133 MDD with sleep disorder) patients and 105 insomnia and 145 healthy controls by SNaPshot SNP technology. 3) To measure further the objective sleep indexes in 25 MDD patients,15 insomnia and 14 healthy controls by PSG equipment.Results:1) There are not significant differences in the genotypic frequency and allele frequency of CLCOK gene rs1801260 or rs11133397 or 3817444 polymorphism in depressed patients and depression with sleep disorder(P＞0.05);there are significant differences in the HAMA total scores in the C/T and T/T genotypic frequency of CLOCK gene rs1801260 in depressed patients(P＜0.05).2) There are not significant differences in the genotypic frequency and allele frequency of CLCOK gene rs1801260 polymorphism in insomnia(P＞0.05);there are significant differences in the allele frequency of CLCOK gene rs11133391 or rs3817444 in insomnia and controls(P＜0.05).3) There are significant differences in onset age,HAMA total scores,HAMD total scores in three genotypes of CLOCK gene rs1801260,rs11133391 and rs3817444 in insomnia and controls(P＜0.05).4) There are significant differences in sleep latency period,the awake number after sleep,the total awakening time(AT),AT/ Total Sleep Time(TST)%in depressions,insomnia and controls(P＜0.01);There are significant differences in TST, sleep efficiency(SE),sleep maintenance(SM),sleep stagel(S1),sleep stage(S2) in depressions,insomnia and controls(P＜0.05).5) The REM time(RT),REM percentage(RT%) in insomnia are significantly lower than those in depressions and controls;The REM latency(RL),awakening numbers(AN) are higher than those in depressions and controls.6) There are significant differences in REM activity(RA),REM Intensity(RI),the first REM time(FRT),the first REM time pencentage(FRT%),REM sleep number(RSN) in depressions,insomnia and controls(P＜0.01);7) There are significant differences in sleep efficiency,actuality sleep time and sleep latency in objective sleep and subjective sleep in depressions(P＜0.05); There are significant differences in sleep efficiency,actuality sleep time and sleep latency in objective sleep and subjective sleep in insomnia(P＜0.05).Conclusions:1) CLOCK gene rs1801260 or rs11133391 or rs3817444 polymorphism are not significantly related with depression(concluding depression with sleep disorder); CLOCK gene is not probably related with depression.2) The allele frequency of CLCOK gene rs11133397 or 3817444 polymorphism are significantly related with insomnia.3) There are significant differences in onset age in three genotypes of CLOCK gene rs1801260,rs11133391 and rs3817444 in insomnia;4) The depressed patients and insomnia have severe sleep disorders;the sleep efficiency and actuality sleep of objective sleep in depression and insomnia are significantly higher than those of subjective sleep,and sleep latency is contrary.5) The REM time(RT),REM percentage(RT%) in insomnia are significantly lower than those in depressions and controls;The REM latency(RL),awakening numbers(AN) are higher than those in depressions and controls;the REM activity(RA),REM Intensity(RI),the first REM time(FRT),the first REM time pencentage(FRT%),REM sleep number(RSN) in depressions are significantly higher than those in insomnia and controls(P＜0.01);6) There are significant differences in polysomnography variablies in different gender in depression and insomnia.