Collagen-induced Arthritis Associated With The Establishment Of Animal Models Of Depression And Intervention Mechanism

OBJECTIVE:To establish rat model of collagen-induced arthritis with depression, the rats were induced arthritis with collagen first,and then were separately raised and put into chronic unpredictable mild stress.And evaluate the effects of intervention bushen huoxue jieyu prescription on rat model of collagen-induced arthritis with depression.METHODS:In experiment one and two,female Wister rats were randomly divided into normal group,model group,arthritis group and depression group.Normal group were raised normaly;model group were separately raised and put into chronic unpredictable mild stress after being induced arthritis with typeâ…¡collagen; arthritis group were raised normaly afer being induced arthritis with typeâ…¡collagen;depression group were separately raised and put into chronic unpredictable mild stress.After modeling,all rats were carried on behavior test,synovial pathological observation,determination of monoamine neurotransmitter in hypothalamus. In experiment three and four, female Wister rats were randomly divided into normal group, model group, western medicine group and chinese medicine group. Normal group were raised normaly;model group,western medicine group and chinese medicine group were separately raised and put into chronic unpredictable mild stress after being induced arthritis with typeâ…¡collagen. After modeling,they received intragastric administration of double distilled water,fluoxetin and bushen huoxue jieyu prescription respectively once a day for 6 weeks from the first day after the establishment of model.After that,the behavioral evaluation were done and monoamine neurotransmitter in hypothalamus were determined.RESULTS:Results of experiment one:The degree of joint swelling and arthritis index of arthritis group and model group were higher than normal group and there was great difference (P< 0.01).In arthritis group and model group,synovial tissue was hyperemia, synovial cells was irregular,inflammatory cells was infiltration,fibrous tissue was hyperplasia,and there was pannus formation with cartilage and bone destruction. In model group and depression group,the amount of sugar-water consumption by the rats,both the crossing time and rearing time in the open-field test,was much less than that by the rats in the normal group (P< 0.01). Results of experiment two:Compared with normal group, the contents of 5-HT,5-HIAA,NA,DA of model group and depression group were much less,and there was great difference (P< 0.01).The results of experiment one and two confirmed that rat model of collagen-induced arthritis with depression was established successfully.Resluts of experiment three:Compared with normal group, the amount of sugar-water consumption of chinese medicine group and western medicine group was much less (P< 0.01),but was much more than model group's (P< 0.01),and there was no difference between chinese medicine group and western medicine group (P> 0.05).Both the crossing time and rearing time of chinese medicine group and western medicine group in the open-field test were higher than model group's (P < 0.01), and there was no difference between chinese medicine group and western medicine group (P> 0.05). Results of experiment four:The contents of 5-HA,5-HIAA,NA and DA of model group decreased significantly, compared with normal group (P< 0.01).The contents of 5-HA and 5-HIAA of chinese medicine group and western medicine group increased,and chinese group increased more,compared with model group (P< 0.01). There was no great difference on the contents of NA between chinese medicine group and western medicine group and model group (P> 0.05).Compared with model group,there was no great difference in western medicine group (P> 0.05),but great difference in chinese group (P< 0.01).CONCLUSION:was an ideal animal model of composite collagen-induced arthritis,chronic unpredictable mild stress and isolated raising to prepare rat model of collagen-induced arthritis with depression for reseaching collagen-induced arthritis with depression.Bushen huoxue jieyu prescription has obvious effect antidepressant for rheumatoid arthritis with depression,and we considered the mechanisms of the treatment of rheumatoid arthritis with depression relating to improving brain neurotransmitters.