Multipotent Adult Progenitor Cells Derived From Bone Marrow Induced Into Pacemaking Cells

Biological pacemaker is a hot issue in cardiovascular research. The major theme of these studies were to stimulate function of the native pacemaker or create a new pacemaker foci by:â‘ gene transfer to existing cardiomyocytes,â‘¡cellular transplantation, orâ‘¢transplantation of a tissue engineered sinus node. However, gene transfer lacks effective vectors and transgene expression is transient. Cellular transplantation is an alternative approach that can overcome some of the aforementioned shortcomings of gene therapy. There are no satisfying cell sources for creating biological pacemakers. The shortcomings include inadaptability to the human body and the limited availability for sinoatrial node cells, fetal cardiomyocytes and genetically modified cardiomyocytes. In conclusion, it is critical to seek a ideal seeding cell for creating biological pacemakers. In this study, MAPCs will be induced differentiation towards cardiac pacemaking cells in vitro by condition medium and coculture. The objective is to provide an new source of cardiac pacemaking cells, which could proliferate quickly and were readily available without immunogenicity, for biological pacemakers. Conditioned Medium can not make MAPCs differentiate into typical pacemaker cells, but have the pacemaker ion channels. MAPCs cocultured with sinus node cells can differentiate into pacemaker-like cells and Cocultured can drive cardiomyocytes beating in vitro.