Supplementing Qi And Activating Blood Circulation Method On Effect Of Retinal Microvascular Lesion In Rats With Diabetes

Purpose:Diabetic retinopathy (DR) is one of the most common and serious microvascular complication of diabetes mellitus (DM),and the main reason leading to blindness as well. The cause of the disease has yet to be determined. In addition, there are no definite and effective measures to prevent it in the early stage.To study the cause of the disease and to find effective drug are the hot spot in each country's ophthalmology. The research of the mechanism involves in biochemistry,molecular biology and blood dyscrasia,but it's center still is high blood sugar which causes a series changes of ischemia and hypoxia. Thanks to its peculiar theory and treatment, traditional Chinese medicine has achieved some success in the prevention and treatment of DR. According to the principle of curing illness from the origin.We select the traditional Chinese medicine QiShenYiQi Gutta Pills(QGP) to YiQiHuoXue Therefore,an attempt is made in this paper to construct a rat model of DM to made a further research into the mechanism of QiShenYiQi Gutta Pills in the prevension of DR in its early stage.Methods:The diabetic rat model was established by one-time injection of streptozotozin (STZ,65mg/kg). All SD rats (n=82) were randomly divided into the normal group,model group,control group and QGP group. The extractum of QGP (0.2g/(kg.d)) was administrated intragastrically and the same as the extractum of DuoBeiSi (0.2g/(kg.d)).After 10 months, the rats were killed and the eyeballs were collected for the observation of retina. The expression of VEGF and ICAM-1 in retinal microvascular were detected by using immunohistochemistry method,the expression of VEGF,AGEs and PEDF in retina were detected by using immunohistochemistry method, and ICAM-1 mRNA,VEGF mRNA,RAGE mRNA and PEDF mRNA were examined by using reverse transcription-polymerase chain reaction(RT-PCR).The blood samples were collected from rats for detecting the serum content of VEGF and ICAM-1. Immunological histochemistry assay was used for quantitative analysis. SPSS was used for statistical analysis.Reaults:(1)The content of glucose were higher in the model group,control group and QGP group than that in the normal group, (p<0.01), and were lower in the control group and QGP group than that in the model group,but had no statistical significantly. (2) The weight were lower in the model group,control group and QGP group than that in the normal group, (p<0.01), and were higher in the control group and QGP group than that in the model group,but had no statistical significantly. (3) The serum content of VEGF and ICAM-1 were higher in the model group,control group and QGP group than that in the normal group,and were lower in the control group and QGP group than that in the model group,but had no statistical significantly. (4)Ten months later, DM rats displayed some early symptoms of DR, such as extensive pericyte loss, endothelial cell proliferation, pericyte ghost, and acelluar capillary. After treatment of QGP, the retinopathy has improved remarkably. (5) The expressions of retinal capillary VEGF and ICAM-1 were higher significantly in the model group than those in the normal group,and were lower significantly in the control group and QGP group than that in the model group with a significant difference(p<0.01). (6) The expressions of retinal VEGF and AGEs were higher significantly in the model group than those in the normal group,and were lower significantly in the control group and QGP group than those in the model group with a significant difference(p<0.01). The expression of retinal PEDF was lower significantly in the model group than that in the normal group,and was higher significantly in the control group and QGP group than that in the model group with a significant difference(p<0.01). (7) The expressions of retinal VEGFmRNA,ICAM-1 mRNA and RAGEmRNAwere higher significantly in the model group than those in the normal group,and were lower significantly in the control group and QGP group than those in the model group with a significant difference(p<0.01). The expression of retinal PEDFmRNA was lower significantly in the model group than that in the normal group,and was higher significantly in the control group and QGP group than that in the model group with a significant difference(p<0.01).Conclusion:YiQiHuoXue method (QiShenYiQi Gutta Pills)can delay the occurrence and development of diabetic retinopathy in rats through decreasing the expressions of retinal VEGF VEGFmRNA,ICAM-1,ICAM-1 mRNA,AGEs and RAGEmRNA, and increasing the expressions of retinal PEDF and PEDFmRNA.