Effect Of Transforming Growth Factor-β1 Gene Transferring On Adipose Derived Stem Cells

Objective:On the basis of the fact that TGF-βinducing differentiation of ADSCs to cartilaginous tissues, we use transgenic technology to modificate ADSCs with TGF-β1 gene to study effect of TGF-β1 on ADSCs differentiation and elucidate its value to be seed cells for cartilage tissue engineering.Methods:Use recombinant TGF-β1 adenovirus to transfect ADSCs,we study effect of TGF-β1 in ADSCs on inducing differentiation of ADSCs to be cartilaginous tissues.Results:1.RT-PCR shows that expression of CollagenⅠ,CollagenⅡ,CollagenⅩ,Aggrecan in TGF-β1 gene transferring ADSCs increased significantly,while ALP, PPARγ2 didn t change.2.Expression of GAG in TGF-β1 gene transferring ADSCs increased as transfection time going on.On the fifth and tenth days, the GAG content in ADSCs increased significantly compared with the control(p<0.01). GAG content on the tenth day increased significantly compared with the fifth day(p<0.01).3. CollagenⅡand Aggrecan staining were positive in TGF-β1 gene transferring ADSCs.Conclusions:1.Expression of CollagenⅠ,CollagenⅡ,CollagenⅩ,Aggrecan in TGF-β1 gene transferring ADSCs increased significantly,inducing differentiation of ADSCs to cartilaginous tissues.2.TGF-β1 gene transferring ADSCs have the value to be seed cells for cartilage tissue engineering.