The Relationship Between Legumain Expression Of Human Orbital Rhabdomyosarcoma And Tumor Invasion

Objective1 To investigate the expression of legumain in human orbital rhabdomyosarcoma as well as their relation with biological behaviour of human orbital rhabdomyosarcoma. And analyse the independent clinical factors that affected the survival of patients with rhabdomyosarcoma.2 To detect the expression of legumain and a-SMA in RD cell line stimulated with different concentration of TGF-βand invest the effect of legumain in RD cell line in order to provide new stratagies in tumor treatment.Methods1 The expression of legumain was evaluated in the 56 operative samples of human orbital rhabdomyosarcoma by immunohistochemistry and study the relationship between legumain expression and clinical factors including age, sex, histological type, tumor size, eyelid involved or not and prognosis of patients.2 The expression of legumain was detected by immunofluorescence. The expression of legumain and a-SMA in RD cell line stimulated with different concentration of TGF-βwere detected through RT-PCR and Western-blot.Results1 High expression of legumain were detected in 25 samples of human orbital rhabdomyosarcoma. The expression levels of legumain and the independent clinical factors such as histological type, tumor size, eyelid involved could affect survival of patients with rhbadomyosarcoma.2 The expression level of legumain and a-SMA in RD cell line stimulated with 2.5ng/ml of TGF-βwere higher than that of normal control group while stimulated with 5ng/ml the expression level of legumain and a-SMA in RD cell line were lower than that of normal control group.Conlusion1 The expression of legumain in human orbital rhabdomyosarcoma were high and detecting the expression of legumain may be become biological index for malignancy and prognosis factors of human orbital rhabdomyosarcoma. 2 Patients with low level of legumain exprssion, tumor size≦5cm, embryonal RMS, without eyelid involved had better prognosis.3 The expression level of legumain and a-SMA in RD cell line stimulated with 2.5ng/ml of TGF-βincreased while down regulated in RD cell line stimulated with 5ng/ml of TGF-β. Different concentration of TGF-βhave different effects on prolifaration of RD cell line, legumain may play important roles in the process of RD proliferation and increase the invasion activity of RD cells.