Experiment Studies On Inhibitory Effects Of C-FLIP Antisense Oligonucleotides Loaded Nanoparticles On Human Orbital Rhabdomyosarcoma In Vivo

Objective To investigate the inhibitory effects of c-FLIP antisense oligonucleotides(ASODN) on the suppression of c-FLIP expression and growth of human orbital rhabdomyosarcoma xenograft in nude mice. Methods We established the model of human orbital rhabdomyosarcoma xenograft in nude mice; c-FLIP ASODN NP,c-FLIP ASODN and normal saline(NS) were injected in the tumor, respectively; We observed the anti-tumor effect of c-FLIP ASODN NP and safety on human orbital rhabdomyosarcoma therapy by a series of experiments in vivo. Results The growth of human orbital rhabdomyosarcoma in nude mice was inhibited in the ASODN NP group obviously ( P < 0.05); and c-FLIP is decreased obviously in ASODN NP group and more apoptotic cells are detected . Conclusions c-FLIP ASODN can effectively inhibit the suppression of c-FLIP, and it induces more apoptotic cells and inhibits the growth of tumor. But the ASODN isn't stable in vivo if it don't be wrap up, our research reveals that nanoparticle is a safe and efficient vector to mediated ASODN.