Experimented Study On The Mechanism Of Yishenxujing Decoction In Delaying Aging Of Ovary In Early Stage Of Menopausal Transition Period

Speciality:Gynecology of Traditional Chinese Medicine Author:YuxinhuiTutor:LiLieRongObjects:Probing the Mechanism of aging in early stage of menopausal transition period, and Through establishing the animal model, investigating the mechanism of Yishenxujing decoction in delaying aging of ovary from regulating endocrine secretion and relectting ovarion apoptosis pathway of caspase-depended typle.Theory probing Deficiency of hepatic blood is the typical feature of aging in early stage of menopausal transition period. The speech of aging is closed with kidney. Blood stasis is products of aging, and blood stasis is one of the most important factors for accelerating speed of aging. Professor li lie rong aims directly at the special physiological functions and pathology of the menopausal transition, giving consideration to Liver and kidney. She suggests delaying the speech of female aging by use of traditional Chinese drug in early stage of menopausal transition period, delay the menopause age and reduce taking place of the menopausal syndromes, this is ref lectting for the theory of prevention and cure redisease. Meanwhile, promoting blood flow to avoide the stagnated blood blocking veins and arteries, which reflects the theory of preventting development for the disease.Experiment studyMethods:The experiment chose MT female rats as research object, made experiment with vagina cell fitting the model standard. We distinguished the rast as MT initial stage group and MT advanced stage group depend on the age of rats. we distinguished the rats as MT initial stage model group,MT initial stage treatment group and western medicine group from 24 rats which accord with MT initial stage standard. Then we distinguished the rats as MT advanced stage model group and MT advanced stagetreatment group from 16 rats which accord withMT advanced stage standard. Then chose 8 young rats as the young control group. The rats of MT initial stage model group,MT advansed stage model group and the young control group were gavaged with isotonic Na chloride. The rats of MT initial stage treatment group and MT advanced stagetreatment group were gavaged by gastric perfusion of yishenxujing decoction. The Western medicine group received Mafulong by gastric perfusion. After lavaged for 15 days, drawed blood from abdominal aorta, separated the blood serum. The blood sample were tested to detect serum INHB,FSH,E2. The ovaries were treated by HE and Immune histoehemistry method to observed the morphologe of overies,expression of overy Fas,Cytc,Caspase-12 and Caspase-3.Results:1. INHB level of young group is higher than MT initial stage model group (P<0.01), and INHB level of MT initial stage model group is higher than MT advanced stage model group (P<0.01). INHB level of MT initial stage treatment group is higher than MT initial model group (P<0.01). INHB level of MT advanced stage treatment group hasn't obvious difference with MT advanced stage group (P<0.05).The western medicine group is approach with MT initial stage model group (P>0.05).2. The FSH level of MT initial stage model group is higher than young group (P<0.01), and FSH level of MT advanced stage model group is higher than MT initial stage model group (P<0.01).The young group is approach with MT initial stage model group (P>0.05). FSH level of western medicine group is lower than MT initial stage model group (P<0.01), But is higher than the young group (P<0.05).FSH level of MT advanced stage treatment group is approach with MT advanced stage model group (P>0.05).3. The E2 level of MT initial stage model group is obviously higher than young group (P<0.01).The E2 level of MT advanced stage model group is obviously lower than young group (P<0.01). After using the yishenxujing decoction, E2 level of MT initial stage treatment group is lower than MT initial stage model group (P<0.01), E2 level of MT advanced stage treatment group is higher than MT advanced stage model group (P<0.01).4. The ovarian index of the young group is higher than MT initial stage model group (P<0.01).The ovarian index of MT initial stage model group is higher than MT advanced stage model group (P<0.01), MT initial stage treatment group is higher than MT advanced stage treatment group (P<0.05), MT advanced stage treatment group is a little higher than MT initial stage model group, but there is not statistical significance (P>0.05). The ovarian index of western medicine group is approach with MT initial stage model group (P>0.05). 5.From the light microscope, we can see there is more primary follicle,secondary follicle,sinusoid follicle in overies of MT initial stage group, and the follicles is regular and big. In ovary cortex of MT initial stage model group and western medicine group rats, there are less follicles, follicles'morphous are irregular. Follicles in ovaries of MT advanced stage treatment group and MT advanced stage model group rats are small and irregular.6. Fas expression in ovaries of MT advanced stage model group is higher than MT initial stage model group (P<0.05). And the ovaries Fas expression of young group is lower than MT initial stage model menopausal transition group(P<0.01). After using the yishenxujing decoction, Fas expression in ovaries of MT initial stage treatment group is lower than MT initial stage model group (P<0.05). MT advanced stage treatment group hasn't statistical significance with MT initial stage model group (P>0.05). The ovaries Fas expression of western medicine group is approach with MT initial stage model group (P>0.05)7. Caspase-12 expression in ovaries of MT advanced stage model group is higher than MT initial stage model group (P<0.05). And the ovaries Caspase-12 expression of young group is lower than MT initial stage model group (P<0.01). Caspase-12 expression of MT initial stage treatment group is lower than MT initial stage model group(P<0.05), and apporoach with young group (P>0.05).Expression in ovaries of MT advanced stage expression is lower than MT advanced stage model group(P<0.05), west medicing group is approach with MT initial stage model group (P>0.05).8. Compared with other groups, CytC,Caspase-3 expression in ovaries of young group is the lowest. And the overies CytC,Caspase-3 expression of MT advanced stage model group is higher than MT initial stage model group (P<0.01,P<0.01).CytC,Caspase-3 expression in overies of MT initial stagetreatment group is lower than MT initial stage model group (P<0.01,P<0.05), but is higher than young group (P<0.01,P<0.01). CytC,Caspase-3 expression of MT advanced stage model group is a little lower than MT initial stage model group (P>0.05,P>0.05). The western medicine group is approach with MT initial stage model group (P>0.05).Conclusion:The topic erect the initial stage model of the menopausal transition. It makes the foundation for study the menopausal transition from experimentation. My teacher Lilierong makes the principle of giving consideration to Liver and kidney and promoting blood flow, drows up Yisenxujing decoction, which can delay ovarian apoplexies when heating irregular menstruation in early stage of menopausal transition. Experiment invest indicated, yisenxujing decoction could adjust the ovarian follicle development and adjust the estrogenic hormone fluctuation. Yisenxujing decoction could adjust the estrogenic hormone level and amendment the estrogenic hormone effect, improved the protein synthesis. Yisenxujing decoction has the forward direction action for the reproduction genealogy. Yisenxujing decoction could depress expression of Fas,CytC and Caspase-12, depress the activation and dilivery of ovary's apoptosis pathway of caspase depended typle, so that depress expression of Caspase-3 gene, delaying the proceeding of ovarian aging.